Intralesional injection of vitamin D3, platelet rich plasma versus their combination in treatment of keloid: a clinical, radiological and immunohistochemical study.
- 2025-05-20
- Archives of dermatological research 317(1)
- Nagwa Mohammad Elwan
- Yomna Mazid El-Hamd Neinaa
- Radwa Mostafa Elkhouly
- Raghdah Magdy Dagher
- Shady Fikry Abdel Salaam
- PubMed: 40392350
- DOI: 10.1007/s00403-025-04244-5
Study Design
- Type
- Randomized Controlled Trial (RCT)
- Population
- sixty keloid patients
- Methods
- randomly split into three matched groups; Group I received intralesional vitamin D3 injection; Group II got intralesional PRP; Group III received both alternatively; biweekly until clinical cure or for a maximum of 4 successive sessions
- Duration
- biweekly until clinical cure or for a maximum of 4 successive sessions
Management of keloid is still a challenge. Many treatment modalities are available, yet no definitive treatment protocol exists. Vitamin D3 (Vit. D3) has an anti-inflammatory role in preventing tissue fibrosis. Platelet-rich plasma (PRP) contains plentiful various peptides that have anti-inflammatory and tissue repair activities. The study aimed to assess the safety and efficacy of injecting either Vit. D3 or PRP versus their combination in keloid treatment. There were sixty keloid patients in all, randomly split into three matched groups for this research. Group I received an intralesional vitamin D3 injection; Group II got an intralesional PRP. While group III received both alternatively. All patients received treatment session biweekly until clinical cure or for a maximum of 4 successive sessions. Therapeutic efficacy was assessed by Verbal rating scale (VRS), Vancouver scar scale (VSS), dermoscopic, and ultrasonic examinations. Additionally, H&E and immunohistochemical expression of Caveolin-1 (Cav-1) were studied. Significant improvements in both VSS and VRS of keloid scars were detected in the three groups studied. These findings were significantly higher among patients treated by combined Vit. D3 and PRP followed by Vit. D3 monotherapy, and lastly PRP monotherapy. Parallel dermoscopic and radiological findings were detected confirming the clinical results. In the three groups studied, histopathological examination reported significant reduction in collagen with more compact orientation, and significant increase of Cav-1 immunohistochemical expression in keloid scars after treatment. Intralesional injection of either Vit. D3, PRP or their combination are safe and effective in keloid treatment.
Research Insights
Significant improvements in both VSS and VRS of keloid scars were detected in the three groups studied.
- Effect
- Beneficial
- Effect size
- Moderate
Significant improvements in both VSS and VRS of keloid scars were detected in the three groups studied. These findings were significantly higher among patients treated by combined Vit. D3 and PRP followed by Vit. D3 monotherapy, and lastly PRP monotherapy.
- Effect
- Beneficial
- Effect size
- Moderate
significant increase of Cav-1 immunohistochemical expression in keloid scars after treatment
- Effect
- Beneficial
- Effect size
- Moderate
histopathological examination reported significant reduction in collagen with more compact orientation
- Effect
- Beneficial
- Effect size
- Moderate
Adverse Events Reported
Intralesional injection of either Vit. D3, PRP or their combination are safe and effective in keloid treatment.
- Finding
- Reported