The unsaturated fatty acid of the cell membrane and the accumulation of intracellular glutamic acid as key determinants of freeze-drying tolerance in Bifidobacterium bifidum.
- 2026-03
- Food research international (Ottawa, Ont.) 228
- Shuyu Guo
- Wenrui Zhou
- Bingyong Mao
- Xin Tang
- Qiuxiang Zhang
- Jianxin Zhao
- Wei Chen
- Shumao Cui
- PubMed: 41703885
- DOI: 10.1016/j.foodres.2026.118444
- Low evidence
The addition of Tween 80 or sodium oleate increased membrane unsaturation and freeze-drying survival rate. Additionally, exogenous glutamic acid supplementation enhanced the freeze-drying survival rate of CCFM16 (up to 48.83 ± 2.04%).
- Effect
- Beneficial
- Effect size
- Moderate