Streptococcus thermophilus ST-21 and Improved Detection of Live Microorganisms in Probiotic Formulations

• Triplex viability PCR for simultaneous quantification of Lactobacillus acidophilus, Streptococcus thermophilus, and Bifidobacterium bifidum in live microbial formulations.

  • 2026-02-10
  • Frontiers in microbiology 17
  • • Luca Del Pio
    • Stefania Catone
    • Giulio Pisani
    • Giovanna Franciosa

  • PubMed: 41743130
  • DOI: 10.3389/fmicb.2026.1759060

  1 Insight on

  • Improved Detection of Live Microorganisms in Probiotic Formulations

  • Low evidence

  By combining multiplexing, high-efficiency reagents, and a PMA pretreatment that selectively prevents amplification from non-viable cells, this triplex vPCR method overcomes the limitations of culture-dependents techniques and standard qPCR, improves analytical throughput, reduces variability, ensuring accurate quantification of viable microorganisms.

  Effect

  Beneficial

  Effect size

  Moderate