A TaqMan™-based quantitative PCR screening assay for the probiotic Streptococcus salivarius K12 based on the specific detection of its megaplasmid-associated salivaricin B locus.
- 2020-03
- Journal of Microbiological Methods 170
- PubMed: 31923428
- DOI: 10.1016/j.mimet.2020.105837
Study Design
- Methods
- n/a
Abstract
In order to assess the colonization efficacy of the oral probiotic Streptococcus salivarius K12, a rapid method for specific detection and enumeration of the strain was developed. Here, we describe a two-step TaqMan™ quantitative PCR assay using primer-probe combinations targeting genes of the locus encoding the lantibiotic bacteriocin salivaricin B.
Keywords: Salivaricin B; Streptococcus salivarius K12; TaqMan™ quantitative PCR assay.
Research Insights
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