Accumulation of polyphosphate in Lactobacillus spp. and its involvement in stress resistance.

2014-03
Applied and environmental microbiology 80(5)

PubMed: 24375133
DOI: 10.1128/aem.03997-13

Study Design

Population: 34 strains of Lactobacillus
Methods: Poly-P accumulation was assessed by specific staining and electron microscopy; the ppk gene from Lactobacillus was disrupted in Lactobacillus casei BL23 strain, and resistance to several stresses was tested in the ppk-disrupted strain

Rigorous Journal

Polyphosphate (poly-P) is a polymer of phosphate residues synthesized and in some cases accumulated by microorganisms, where it plays crucial physiological roles such as the participation in the response to nutritional stringencies and environmental stresses. Poly-P metabolism has received little attention in Lactobacillus, a genus of lactic acid bacteria of relevance for food production and health of humans and animals. We show that among 34 strains of Lactobacillus, 18 of them accumulated intracellular poly-P granules, as revealed by specific staining and electron microscopy. Poly-P accumulation was generally dependent on the presence of elevated phosphate concentrations in the culture medium, and it correlated with the presence of polyphosphate kinase (ppk) genes in the genomes. The ppk gene from Lactobacillus displayed a genetic arrangement in which it was flanked by two genes encoding exopolyphosphatases of the Ppx-GppA family. The ppk functionality was corroborated by its disruption (LCABL_27820 gene) in Lactobacillus casei BL23 strain. The constructed ppk mutant showed a lack of intracellular poly-P granules and a drastic reduction in poly-P synthesis. Resistance to several stresses was tested in the ppk-disrupted strain, showing that it presented a diminished growth under high-salt or low-pH conditions and an increased sensitivity to oxidative stress. These results show that poly-P accumulation is a characteristic of some strains of lactobacilli and may thus play important roles in the physiology of these microorganisms.

Research Insights

Supplement	Dose	Health Outcome	Effect Type	Effect Size	Source
Lactobacillus brevis SBC8803	—	Reduced Polyphosphate Accumulation	Harmful	Moderate	View source The constructed ppk mutant showed a lack of intracellular poly-P granules and a drastic reduction in poly-P synthesis.