Administration of Lactococcus lactis strain Plasma induces maturation of plasmacytoid dendritic cells and protection from rotavirus infection in suckling mice
- 2018-03
- International Immunopharmacology 56
- K. Jounai
- Tetsu Sugimura
- Yuji Morita
- Konomi Ohshio
- Daisuke Fujiwara
- PubMed: 29414652
- DOI: 10.1016/j.intimp.2018.01.034
Study Design
- Type
- Clinical Trial
- Population
- Suckling mice
- Methods
- In vivo experiment with oral administration in a mouse model.
- Rigorous Journal
- Animal Study
Abstract
Lactococcus lactis subsp. lactis JCM 5805 (LC-Plasma) is a strain of lactic acid bacteria (LAB) that activates murine and human plasmacytoid dendritic cells (pDCs) to express interferons (IFNs). Oral administration of LC-Plasma drastically decreased fatality levels caused by parainfluenza virus infection in a murine model. In this study, we investigated the anti-viral effects of oral administration of LC-Plasma using a suckling mouse model of rhesus rotavirus (RV) infection. LC-Plasma-fed mice showed improvement in retardation of body weight gain, fecal scores, and a reduction in RV titer in the feces when compared to control mice. The mechanism of anti-viral effects elicited by LC-Plasma administration was investigated using naive mice: in the LC-Plasma -fed mice, lamina propria (LP) pDCs resident in the small intestine were significantly matured and the proportion of pDCs was increased. The expression levels of anti-viral factors induced by IFNs, such as Isg15, Mx1, Oasl2 and Viperin, and an anti-bacterial factor Reg3γ, were up-regulated in the small intestinal epithelial cells (IECs) of LC-Plasma-fed mice. The specific LAB strain may affect the anti-viral immunological profile of IECs via maturation of LP pDCs, leading to protection from RV virus infection in vivo.
Keywords: Lactococcus lactis subsp. lactis JCM 5805; Plasmacytoid dendritic cells; Rotavirus.