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Evidence-Based Supplement Research
Evidence-Based Supplement Research

Study Design

Type
Randomized Controlled Trial (RCT)
Sample size
n = 67
Population
67 children living near a coking plant
Methods
randomized crossover trial; linear mixed-effects model analyses; network and mediation analyses
Blinding
Open-label
Duration
short-term air purification
Recent studies have identified the association between exposure to environmental particulate matter (PM) and upper respiratory tract microbiota or lower respiratory tract metabolites; however, direct evidence linking the respiratory microbiome to metabolomic responses remains limited. This study examines how short-term air purification influences PM-related health outcomes, nasal microbiota, and exhaled breath condensate (EBC) metabolites in children. In a randomized crossover trial involving 67 children living near a coking plant, the linear mixed-effects model analyses showed that PM exposure was associated with changes in vital capacity and heart rate. Moreover, PM exposure reduced the abundance of nasal Rothia, which was closely connected to changes in EBC metabolites, including hexylresorcinol and β-carotene. Network and mediation analyses revealed that the PM-Rothia-metabolite axis may exert a significant impact on cardiopulmonary function, among which β-carotene is likely to mediate the effect of PM on vital capacity. Our findings indicate that short-term air purification can reduce PM-associated cardiopulmonary risks in children by modulating Rothia-dependent metabolic pathways.

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