Ameliorative Effects of Probiotic Lactobacillus paracasei NL41 on Insulin Sensitivity, Oxidative Stress and Beta-Cell Function in a Type 2 Diabetes Mellitus Rat Model.
- 2019-09-12
- Molecular Nutrition & Food Research 63(22)
- Z. Zeng
- Qipeng Yuan
- Rui Yu
- Jinlan Zhang
- Huiqin Ma
- Shangwu Chen
- PubMed: 31433912
- DOI: 10.1002/mnfr.201900457
Abstract
Scope: The present study aims to assess the antidiabetic effect of Lactobacillus paracasei strain NL41 and its potential mechanisms in rats with type 2 diabetes mellitus (T2DM) induced by a high-fat diet and low-dose streptozotocin administration (HFD/STZ).
Methods and results: Eighteen Sprague-Dawley (SD) rats are randomly assigned to three groups: one control, one HFD/STZ model, and one HFD/STZ-Lactobacillus protection group with administration of strain NL41 for 12 weeks. Blood is collected for biochemical parameters analysis and tissue samples for histological analysis. Treatment with strain NL41 results in excellent blood glucose regulation and significantly decreases insulin resistance, and HbA1c, glucagon, and leptin levels, accompanied by remarkable improvement of dyslipidemia and oxidative stress status in the animals. Islets of Langerhans, liver, and kidney are significantly protected in the NL41-treated rats compared to the HFD/STZ-T2DM model rats. Histochemistry shows that strain NL41 inhibits beta-cell loss and alpha-cell expansion, indicating pancreatic islets as the targeted tissues for the primary ameliorative effect of the probiotic strain on HFD/STZ-T2DM rats. Crosstalk between the gut-liver and liver-pancreas endocrine axes is discussed.
Conclusion: Probiotic strain NL41 prevents HFD/STZ-T2DM by decreasing insulin resistance and oxidative stress status, and protecting beta-cell function.
Keywords: Lactobacillus paracasei NL41; antidiabetic effects; blood glucose; probiotics; type 2 diabetes mellitus.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus casei SD-5842 | Improved Insulin Sensitivity | Beneficial | Large |
| Lactobacillus casei SD-5842 | Preserved Islet Function | Beneficial | Large |
| Lactobacillus casei SD-5842 | Reduced Hepatic Oxidative Stress | Beneficial | Large |
| Lactobacillus paracasei | Improved Insulin Sensitivity | Beneficial | Large |
| Lactobacillus paracasei | Preserved Islets of Langerhans | Beneficial | Large |
| Lactobacillus paracasei | Prevention of Beta-Cell Loss | Beneficial | Large |
| Lactobacillus paracasei | Reduced Blood HbA1c Levels | Beneficial | Large |
| Lactobacillus paracasei | Reduced Blood Leptin Levels | Beneficial | Large |
| Lactobacillus paracasei | Reduced Oxidative Stress | Beneficial | Large |
| Lactobacillus paracasei 431 | Improved Blood Glucose Levels | Beneficial | Large |
| Lactobacillus paracasei 431 | Improved Insulin Sensitivity | Beneficial | Large |
| Lactobacillus paracasei 431 | Improved Lipid Profile | Beneficial | Large |
| Lactobacillus paracasei 431 | Preserved Islet Function | Beneficial | Large |
| Lactobacillus paracasei 431 | Reduced Blood HbA1c Levels | Beneficial | Large |
| Lactobacillus paracasei 431 | Reduced Hepatic Oxidative Stress | Beneficial | Large |
| Lactobacillus paracasei DSM 13434 | Improved Blood Glucose Levels | Beneficial | Large |
| Lactobacillus paracasei DSM 13434 | Improved Insulin Sensitivity | Beneficial | Large |
| Lactobacillus paracasei DSM 13434 | Preserved Islet Function | Beneficial | Large |
| Lactobacillus paracasei DSM 13434 | Reduced Hepatic Oxidative Stress | Beneficial | Large |
| Lactobacillus paracasei HA-196 | Improved Insulin Sensitivity | Beneficial | Large |
| Lactobacillus paracasei HA-196 | Improved Lipid Profile | Beneficial | Large |
| Lactobacillus paracasei HA-196 | Reduced Hepatic Oxidative Stress | Beneficial | Large |
| Lactobacillus paracasei HA-196 | Regulated Blood Glucose Levels | Beneficial | Large |
| Lactobacillus paracasei IMC 502 | Improved Blood Glucose Levels | Beneficial | Large |
| Lactobacillus paracasei IMC 502 | Improved Dyslipidemia | Beneficial | Large |
| Lactobacillus paracasei IMC 502 | Improved Insulin Sensitivity | Beneficial | Large |
| Lactobacillus paracasei IMC 502 | Preserved Beta-Cell Function | Beneficial | Large |
| Lactobacillus paracasei IMC 502 | Reduced Blood HbA1c Levels | Beneficial | Large |
| Lactobacillus paracasei IMC 502 | Reduced Glucagon Levels | Beneficial | Large |
| Lactobacillus paracasei IMC 502 | Reduced Hepatic Oxidative Stress | Beneficial | Large |
| Lactobacillus paracasei IMC 502 | Reduced Leptin Levels | Beneficial | Large |
| Lactobacillus paracasei LPC09 | Improved Blood Glucose Control | Beneficial | Large |
| Lactobacillus paracasei LPC09 | Improved Insulin Resistance | Beneficial | Large |
| Lactobacillus paracasei LPC09 | Improved Insulin Sensitivity | Beneficial | Large |
| Lactobacillus paracasei LPC09 | Preserved Islet Function | Beneficial | Large |
| Lactobacillus paracasei LPC09 | Reduced Hepatic Oxidative Stress | Beneficial | Large |
| Lactobacillus paracasei LPC09 | Reduction of Diabetes-Induced Organ Damage | Beneficial | Large |
| Lactobacillus paracasei MCC1849 | Improved Beta-Cell Function | Beneficial | Large |
| Lactobacillus paracasei MCC1849 | Improved Glucose Regulation | Beneficial | Large |
| Lactobacillus paracasei MCC1849 | Improved Insulin Sensitivity | Beneficial | Large |
| Lactobacillus paracasei MCC1849 | Reduced Hepatic Oxidative Stress | Beneficial | Large |
| Lactobacillus paracasei subsp. paracasei UALpc-04 | Improved Blood Glucose Levels | Beneficial | Large |
| Lactobacillus paracasei subsp. paracasei UALpc-04 | Improved Insulin Sensitivity | Beneficial | Large |
| Lactobacillus paracasei subsp. paracasei UALpc-04 | Improved Lipid Profile | Beneficial | Large |
| Lactobacillus paracasei subsp. paracasei UALpc-04 | Preserved Islet Function | Beneficial | Large |
| Lactobacillus paracasei subsp. paracasei UALpc-04 | Reduced Alpha-cell Expansion | Beneficial | Moderate |
| Lactobacillus paracasei subsp. paracasei UALpc-04 | Reduced Beta-Cell Loss | Beneficial | Large |
| Lactobacillus paracasei subsp. paracasei UALpc-04 | Reduced Blood HbA1c Levels | Beneficial | Large |
| Lactobacillus paracasei subsp. paracasei UALpc-04 | Reduced Blood Leptin Levels | Beneficial | Large |
| Lactobacillus paracasei subsp. paracasei UALpc-04 | Reduced Hepatic Oxidative Stress | Beneficial | Large |
| Lactobacillus paracasei subsp. paracasei UALs-04 | Preserved Islet Function | Beneficial | Large |
| Lactobacillus paracasei subsp. paracasei UALs-04 | Reduced Blood Glucose Levels | Beneficial | Large |
| Lactobacillus paracasei subsp. paracasei UALs-04 | Reduced Glucagon Levels | Beneficial | Large |
| Lactobacillus paracasei subsp. paracasei UALs-04 | Reduced Leptin Levels | Beneficial | Large |
| Lactobacillus paracasei UALpc-04 | Improved Insulin Sensitivity | Beneficial | Large |
| Lactobacillus paracasei UALpc-04 | Improved Lipid Profile | Beneficial | Large |
| Lactobacillus paracasei UALpc-04 | Preserved Islet Function | Beneficial | Large |
| Lactobacillus paracasei UALpc-04 | Reduced Blood HbA1c Levels | Beneficial | Large |
| Lactobacillus paracasei UALpc-04 | Reduced Hepatic Oxidative Stress | Beneficial | Large |
| Lactobacillus paracasei VPro 224 | Improved Blood Glucose Levels | Beneficial | Large |
| Lactobacillus paracasei VPro 224 | Improved Insulin Sensitivity | Beneficial | Large |
| Lactobacillus paracasei VPro 224 | Improved Lipid Profile | Beneficial | Large |
| Lactobacillus paracasei VPro 224 | Preserved Islets of Langerhans | Beneficial | Large |
| Lactobacillus paracasei VPro 224 | Reduced Alpha-cell Expansion | Beneficial | Moderate |
| Lactobacillus paracasei VPro 224 | Reduced Beta-Cell Loss | Beneficial | Large |
| Lactobacillus paracasei VPro 224 | Reduced Blood HbA1c Levels | Beneficial | Large |
| Lactobacillus paracasei VPro 224 | Reduced Hepatic Oxidative Stress | Beneficial | Large |