Analysis of the metabolic process of sugarcane juice fermented by Leuconostoc mesenteroides and identification of exopolysaccharides.
- 2025-11
- Food research international (Ottawa, Ont.) 220
- PubMed: 41074314
- DOI: 10.1016/j.foodres.2025.117098
Study Design
- Methods
- Sugarcane juice as substrate for EPS production by Leuconostoc mesenteroides DRP105; maximal EPS yield at 60 h; structural and metabolomic analysis.
Exopolysaccharides (EPS) have garnered significant attention due to their versatile functional attributes and broad-spectrum applications in the food, pharmaceutical, and cosmetic industries. Sugarcane juice was employed as a cost-effective substrate for EPS production by Leuconostoc mesenteroides DRP105. The strain achieved a maximal EPS yield of 42.63 ± 0.56 mg/mL at 60 h, with a molecular weight (Mw) of 6.458 × 105 Da. Structural analysis revealed that the EPS consisted exclusively of glucose monomers, predominantly linked via α-(1,6)- and α-(1,3)-glycosidic linkages, forming a smooth, sheet-like architecture with porous amorphous morphology. The EPS exhibited exceptional solubility, remarkable thermal stability, and potent bioactivities, including antioxidant, emulsifying, and metal-chelating properties. Metabolomic analysis further unveiled a transition from sucrose-specific utilization to diversified carbohydrate metabolism. This metabolic shift is hypothesized to facilitate adaptation to acid stress and maintain cellular viability, thereby sustaining EPS biosynthesis. Collectively, these findings benefit our understanding of EPS biosynthetic mechanisms and present a sustainable, cost-effective strategy to boost industrial production efficiency while harnessing agricultural by-products.
Research Insights
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