Anti-tumor potential of cell free culture supernatant of Lactobacillus rhamnosus strains isolated from human breast milk.

2019-09
Food Research International 123
- M. R. Riaz Rajoka
- Haobin Zhao
- H. Mehwish
- Nan Li
- Yao Lu
- Ziyang Lian
- D. Shao
- M. Jin
- Qi Li
- Liqing Zhao
- Junling Shi

PubMed: 31284979
DOI: 10.1016/J.FOODRES.2019.05.002

Study Design

Population: Cervix cancer cells (HeLa)
Methods: In vitro experiment

Abstract

Lactobacilli rhamnosus has been characterized as a probiotic and plays an important role in human health by stimulating the supplement of nutrients, preventing the colonization of pathogens, and influencing the immune system. This study investigated the anticancer activity of the three Lactobacillus rhamnosus strains SHA111, SHA112, and SHA113 isolated from human breast milk. The cell-free supernatant of a liquid culture of the three strains showed excellent antioxidant activities against DPPH free radicals, superoxide anion radicals, and hydroxyl radicals; furthermore, significant anticancer activity was found on cervix cancer cells (HeLa) via cytotoxicity and induction of apoptosis. RT-qPCR and western blot analysis showed the induction of apoptosis was achieved via the up-regulation of BAD, BAX, Caspase3, Caspase8, Caspase9, and down-regulation of BCL-2 genes in HeLa cells. The results suggest that these strains have potential anticancer capability.

Keywords: Antioxidant; Antitumor; Apoptosis; Lactobacillus; Probiotics.

Research Insights

Lactobacillus rhamnosus DSM33560→Increased Anticancer Activity
significant anticancer activity was found on cervix cancer cells (HeLa) via cytotoxicity and induction of apoptosis.
Effect
Beneficial
Effect size
Large
Lactobacillus rhamnosus DSM33560→Increased Antioxidant Activity
The cell-free supernatant of a liquid culture of the three strains showed excellent antioxidant activities against DPPH free radicals, superoxide anion radicals, and hydroxyl radicals.
Effect
Beneficial
Effect size
Large
Lactobacillus rhamnosus MAK79L08R→Increased Antioxidant Activity
The cell-free supernatant of a liquid culture of the three strains showed excellent antioxidant activities against DPPH free radicals, superoxide anion radicals, and hydroxyl radicals.
Effect
Beneficial
Effect size
Large
Lactobacillus rhamnosus MAK79L08R→Induced Apoptosis
RT-qPCR and western blot analysis showed the induction of apoptosis was achieved via the up-regulation of BAD, BAX, Caspase3, Caspase8, Caspase9, and down-regulation of BCL-2 genes in HeLa cells.
Effect
Beneficial
Effect size
Large
Lactobacillus rhamnosus R0343ND→Increased Anticancer Activity
significant anticancer activity was found on cervix cancer cells (HeLa) via cytotoxicity and induction of apoptosis.
Effect
Beneficial
Effect size
Large
Lactobacillus rhamnosus R0343ND→Increased Antioxidant Activity
The cell-free supernatant of a liquid culture of the three strains showed excellent antioxidant activities against DPPH free radicals, superoxide anion radicals, and hydroxyl radicals.
Effect
Beneficial
Effect size
Large
Lactobacillus rhamnosus R0343ND→Induced Apoptosis
RT-qPCR and western blot analysis showed the induction of apoptosis was achieved via the up-regulation of BAD, BAX, Caspase3, Caspase8, Caspase9, and down-regulation of BCL-2 genes in HeLa cells.
Effect
Beneficial
Effect size
Large
Lactobacillus rhamnosus VPro 11→Increased Anticancer Activity
significant anticancer activity was found on cervix cancer cells (HeLa) via cytotoxicity and induction of apoptosis.
Effect
Beneficial
Effect size
Large
Lactobacillus rhamnosus VPro 11→Increased Antioxidant Activity
The cell-free supernatant of a liquid culture of the three strains showed excellent antioxidant activities against DPPH free radicals, superoxide anion radicals, and hydroxyl radicals.
Effect
Beneficial
Effect size
Large
Lactobacillus rhamnosus VPro 11→Induced Apoptosis
RT-qPCR and western blot analysis showed the induction of apoptosis was achieved via the up-regulation of BAD, BAX, Caspase3, Caspase8, Caspase9, and down-regulation of BCL-2 genes in HeLa cells.
Effect
Beneficial
Effect size
Large