Anti-tumor potential of cell free culture supernatant of Lactobacillus rhamnosus strains isolated from human breast milk.
- 2019-09
- Food Research International 123
- M. R. Riaz Rajoka
- Haobin Zhao
- H. Mehwish
- Nan Li
- Yao Lu
- Ziyang Lian
- D. Shao
- M. Jin
- Qi Li
- Liqing Zhao
- Junling Shi
- PubMed: 31284979
- DOI: 10.1016/J.FOODRES.2019.05.002
Abstract
Lactobacilli rhamnosus has been characterized as a probiotic and plays an important role in human health by stimulating the supplement of nutrients, preventing the colonization of pathogens, and influencing the immune system. This study investigated the anticancer activity of the three Lactobacillus rhamnosus strains SHA111, SHA112, and SHA113 isolated from human breast milk. The cell-free supernatant of a liquid culture of the three strains showed excellent antioxidant activities against DPPH free radicals, superoxide anion radicals, and hydroxyl radicals; furthermore, significant anticancer activity was found on cervix cancer cells (HeLa) via cytotoxicity and induction of apoptosis. RT-qPCR and western blot analysis showed the induction of apoptosis was achieved via the up-regulation of BAD, BAX, Caspase3, Caspase8, Caspase9, and down-regulation of BCL-2 genes in HeLa cells. The results suggest that these strains have potential anticancer capability.
Keywords: Antioxidant; Antitumor; Apoptosis; Lactobacillus; Probiotics.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus rhamnosus | Increased Anticancer Activity | Beneficial | Large |
| Lactobacillus rhamnosus | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus rhamnosus DSM 6594 | Increased Anticancer Activity | Beneficial | Large |
| Lactobacillus rhamnosus DSM 6594 | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus rhamnosus DSM33560 | Increased Anticancer Activity | Beneficial | Large |
| Lactobacillus rhamnosus DSM33560 | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus rhamnosus HA-11 | Cytotoxicity Against Cervical Cancer Cells | Beneficial | Large |
| Lactobacillus rhamnosus HA-11 | Increased Apoptosis in Cancer Cells | Beneficial | Large |
| Lactobacillus rhamnosus HA-111 | Increased Anticancer Activity | Beneficial | Large |
| Lactobacillus rhamnosus HA-111 | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus rhamnosus HA-114 | Increased Anticancer Activity | Beneficial | Large |
| Lactobacillus rhamnosus HA-114 | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus rhamnosus LBV96 | Increased Anticancer Activity | Beneficial | Large |
| Lactobacillus rhamnosus LBV96 | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus rhamnosus LBV96 | Increased Apoptosis in Cancer Cells | Beneficial | Large |
| Lactobacillus rhamnosus LR06 | Increased Anti-Tumor Activity | Beneficial | Large |
| Lactobacillus rhamnosus LR06 | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus rhamnosus LR06 | Increased Apoptosis in Cancer Cells | Beneficial | Large |
| Lactobacillus rhamnosus MAK79L08R | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus rhamnosus MAK79L08R | Induced Apoptosis | Beneficial | Large |
| Lactobacillus rhamnosus MAK79L08R | Reduced Cancer Cell Viability | Beneficial | Large |
| Lactobacillus rhamnosus PXN 54 | Increased Anticancer Activity | Beneficial | Large |
| Lactobacillus rhamnosus PXN 54 | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus rhamnosus PXN 54 | Induced Apoptosis | Beneficial | Large |
| Lactobacillus rhamnosus R0011 | Increased Anticancer Activity | Beneficial | Large |
| Lactobacillus rhamnosus R0011 | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus rhamnosus R0011 | Induced Apoptosis | Beneficial | Large |
| Lactobacillus rhamnosus R0343ND | Increased Anticancer Activity | Beneficial | Large |
| Lactobacillus rhamnosus R0343ND | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus rhamnosus R0343ND | Induced Apoptosis | Beneficial | Large |
| Lactobacillus rhamnosus UALr-18 | Increased Cancer Cell Apoptosis | Beneficial | Large |
| Lactobacillus rhamnosus UALr-18 | Reduced Cancer Cell Viability | Beneficial | Large |
| Lactobacillus rhamnosus VPro 11 | Increased Anticancer Activity | Beneficial | Large |
| Lactobacillus rhamnosus VPro 11 | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus rhamnosus VPro 11 | Induced Apoptosis | Beneficial | Large |