Anti-viral Effect of Bifidobacterium adolescentis against Noroviruses
- 2016-06-08
- Frontiers in Microbiology 7
- Dan Li
- A. Breiman
- J. Le Pendu
- M. Uyttendaele
- PubMed: 27375585
- DOI: 10.3389/fmicb.2016.00864
Abstract
This study aims to investigate the effect of Bifidobacterium adolescentis against noroviruses (NoVs). Murine norovirus-1 (MNV-1) used as a surrogate was detected by plaque assay and RT-qPCR. Human NoV virus like particles (VLPs) were detected by cell-binding assay. It was shown that the presence of B. adolescentis could inhibit the multiplication of MNV-1 on RAW 264.7 cells within 48 h of co-incubation period at 37°C. This inhibition did not occur at the viral binding stage, as no difference was observed in MNV-1 genomic copies collected from washed RAW 264.7 cells without and with B. adolescentis after co-incubation for 1 h at room temperature. Meanwhile, the presence of B. adolescentis decreased the binding of human NoV GI.1 VLPs to both Caco-2 cells and HT-29 cells, while no reduction was induced for the binding of human NoV GII.4 VLPs to Caco-2 cells.
Keywords: Bifidobacterium adolescentis; Norovirus; mechanisms; probiotics.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Bifidobacterium adolescentis iVS-1 | Reduced Norovirus Multiplication | Beneficial | Moderate |
| Bifidobacterium adolescentis iVS-1 | Reduced Viral Binding | Beneficial | Small |