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Antigingivitis and Antiplaque Effects of Oral Probiotic Containing the Streptococcus salivarius M18 Strain: A Randomized Clinical Trial

  • 2023-09-06
  • Nutrients 15(18)
    • K. Babina
    • Dilara Salikhova
    • V. Doroshina
    • Irina Makeeva
    • A. Zaytsev
    • Matvey Uvarichev
    • M. Polyakova
    • N. Novozhilova


We aimed to assess the effect of oral probiotic containing the Streptococcus salivarius M18 strain on gingival inflammation, bleeding on probing, and oral biofilm. Sixty-one consenting participants aged between 18 and 25 with gingivitis were recruited in this double-blind, parallel-group study and randomly divided into the probiotic group (n = 31) and the placebo group (n = 30). Fifty-seven participants completed the entire study protocol, 27 in the probiotic group and 30 in the placebo group. The outcomes were assessed after 4 weeks of intervention and 4 weeks of follow-up. There was a significant decrease in the Gingival Index, with the effect size of 0.58 [95%CI 0.05-1.10], and Turesky modification of the Quigley and Hein Plaque Index, with the effect size of 0.55 [95%CI: 0.02-1.07], in the probiotic group after the intervention. However, after a 4-week follow-up, the only significant treatment outcome was improved gingival condition according to the Gingival Index. The Gingival Bleeding Index also decreased significantly in the probiotic group after the intervention period; after the follow-up, this parameter did not differ significantly in both groups from the baseline values. In the placebo group, there were no significant improvements in the assessed parameters throughout this study. No serious side effects were registered. Within the limitations of this study, we conclude that the use of oral probiotic containing the Streptococcus salivarius M18 strain resulted in a significant improvement in gingival condition and oral hygiene level in young adults with gingivitis. Trial registration NCT05727436. Funding: none.

Keywords: Streptococcus salivarius M18; dental plaque; gingivitis; probiotics.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Streptococcus salivariusImproved Gingival BleedingBeneficial
Streptococcus salivariusImproved Gingival HealthBeneficial
Streptococcus salivariusReduced Plaque LevelsBeneficial
Streptococcus salivarius M18Improved Gingival HealthBeneficial
Streptococcus salivarius M18Reduced Gingival BleedingBeneficial

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