Antiviral effects of Lactobacillus crispatus against HSV‐2 in mammalian cell lines
- 2018-03
- Journal of the Chinese Medical Association 81(3)
- E. Mousavi
- M. Makvandi
- A. Teimoori
- A. Ataei
- S. Ghafari
- A. Samarbaf-zadeh
- PubMed: 29037754
- DOI: 10.1016/j.jcma.2017.07.010
Study Design
- Methods
- In vitro experiment
- Rigorous Journal
Abstract
Background: Herpes simplex virus type 2 (HSV-2) infectious disease is one of the most common viral sexually transmitted diseases. As regards, vaginal lactobacilli play an important role in protecting host against the urogenital pathogens; here we assessed the potential antiviral activity of Lactobacillus crispatus against HSV-2 infection in vitro.
Methods: Both Vero and HeLa cell lines were treated by L. crispatus before, during and after HSV-2 infection. The pre-incubation assay was also performed for the evaluating of virus adsorption by L. crispatus. Virus titer reduction in each stage was determined by a plaque reduction assay.
Results: L. crispatus significantly decreased the infectivity of the HSV-2 in initial steps on both cell lines; however, no significant inhibition was ascertained during adsorption and multiplication process. The lactobacilli adhere on Vero cells two-fold stronger than HeLa and subsequently protect the Vero cells nearly 2.5 fold higher than HeLa cell against the virion. Co-incubation of HSV-2 with bacterial cells prior to virus inoculation significantly decreased the virus titer.
Conclusion: L. crispatus appears to inhibit the entry of the virus into cells by trapping HSV-2 particles. In addition, formation of L. crispatus microcolonies in the cell surface could block HSV-2 receptors and prevent viral entry to cells in initial infection steps.
Keywords: Antiviral activity; HSV-2; HeLa cell line; Lactobacillus crispatus.
Research Insights
The lactobacilli adhere on Vero cells two-fold stronger than HeLa and subsequently protect the Vero cells nearly 2.5 fold higher than HeLa cell against the virion.
- Effect
- Beneficial
- Effect size
- Moderate
L. crispatus significantly decreased the infectivity of the HSV-2 in initial steps on both cell lines; however, no significant inhibition was ascertained during adsorption and multiplication process.
- Effect
- Beneficial
- Effect size
- Moderate
Co-incubation of HSV-2 with bacterial cells prior to virus inoculation significantly decreased the virus titer.
- Effect
- Beneficial
- Effect size
- Moderate
L. crispatus appears to inhibit the entry of the virus into cells by trapping HSV-2 particles.
- Effect
- Beneficial
- Effect size
- Moderate
The lactobacilli adhere on Vero cells two-fold stronger than HeLa and subsequently protect the Vero cells nearly 2.5 fold higher than HeLa cell against the virion.
- Effect
- Beneficial
- Effect size
- Moderate
L. crispatus significantly decreased the infectivity of the HSV-2 in initial steps on both cell lines; however, no significant inhibition was ascertained during adsorption and multiplication process.
- Effect
- Beneficial
- Effect size
- Moderate
Co-incubation of HSV-2 with bacterial cells prior to virus inoculation significantly decreased the virus titer.
- Effect
- Beneficial
- Effect size
- Moderate