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Application of Lactobacillus reuteri B1/1 (Limosilactobacillus reuteri) Improves Immunological Profile of the Non-Carcinogenic Porcine-Derived Enterocytes

  • 2023-04-27
  • Life 13(5)
    • V. Karaffová
    • J. Teleky
    • Maša Pintarič
    • T. Langerholc
    • D. Mudroňová
    • Erik Hudec
    • Z. Ševčíková

Abstract

In our previous studies, Lactobacillus reuteri B1/1, which was renamed Limosilactobacillus reuteri (L. reuteri), was able to modulate the production of pro-inflammatory cytokines and other components of the innate immune response in vitro and in vivo. In this study, we evaluated the effect of Lactobacillus reuteri B1/1 in two concentrations (1 × 107 and 1 × 109 CFU) on the metabolic activity, adherence ability and relative gene expression of pro-inflammatory interleukins (IL-1β, IL-6, IL-8, IL-18), lumican and olfactomedin 4 produced by non-carcinogenic porcine-derived enterocytes (CLAB). CLAB cells were cultured in a 12-well cell culture plate at a concentration of 4 × 105 cells/well in DMEM medium in a controlled humidified atmosphere for 48 h. A 1 mL volume of each probiotic bacterial suspension was added to the CLAB cells. Plates were incubated for 2 h and 4 h. Our results revealed that L. reuteri B1/1 was able to adhere to CLAB cells in sufficient numbers in both concentrations. In particular, the concentration of 109_L. reuteri_ B1/1 allowed to modulate the gene expression of pro-inflammatory cytokines, as well as to increase the metabolic activity of the cells. In addition, administration of L. reuteri B1/1 in both concentrations significantly stimulated gene expression for both proteins in the CLAB cell line after 4 h of incubation.

Keywords: CLAB cells; Lactobacillus reuteri B1/1; metabolic activity; pro-inflammatory cytokine.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Limosilactobacillus reuteri 1E1Improved Adherence AbilityBeneficial
Moderate
Limosilactobacillus reuteri 1E1Improved Metabolic FunctionBeneficial
Moderate
Limosilactobacillus reuteri 1E1Increased Pro-inflammatory Cytokine ReleaseBeneficial
Moderate
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