Assessment of Probiotic Properties of Lactobacillus salivarius Isolated From Chickens as Feed Additives

2020-07-17
Frontiers in Veterinary Science 7
- Jian Wang
- M. Ishfaq
- Yuesheng Guo
- Chunli Chen
- Jichang Li

PubMed: 32766298
DOI: 10.3389/fvets.2020.00415

Study Design

Type: Randomized Controlled Trial (RCT)
Population: White leghorn chickens
Methods: Controlled experimental study

Animal Study

Abstract

The continued use of sub-therapeutic antibiotics as feed additives in the poultry industry improved health and growth performance. However, the resulting antibiotic resistance increasingly becomes a major threat to public health. Probiotics are promising alternatives for the antibiotics used in poultry industry. The aim of this study was to evaluate the probiotic properties of Lactobacillus salivarius as feed additive in chickens. White leghorn chickens were randomly assigned to experimental groups. Effects of Lactobacillus salivarius supplementation on growth performance, resistance to Escherichia coli O78 challenge and heat-stress, and immune response after vaccinated with attenuated infectious bursal disease virus (IBDV) vaccine were determined. The results showed that Lactobacillus salivarius supplementation improved growth performance, such as weight and longer shank length, increased relative weights of the immune organs and decreased concentrations of odor-causing compounds. In addition, Lactobacillus salivarius supplementation alleviated organ injury caused by Escherichia coli O78 challenge and heat stress. Furthermore, Lactobacillus salivarius results in enhanced immune response after IBDV vaccine immunization, enhanced specific antibody and IFN-γ production, and lymphocyte proliferation. Our results revealed a tremendous potential of Lactobacillus salivarius as antibiotics' substitute in poultry production.

Keywords: Escherichia coli; Lactobacillus salivarius; growth performance; heat stress; oxidative stress.

Research Insights

Lactobacillus salivarius SD-5208→Improved Immune Response
Lactobacillus salivarius results in enhanced immune response after IBDV vaccine immunization, enhanced specific antibody and IFN-γ production, and lymphocyte proliferation.
Effect
Beneficial
Effect size
Large
Lactobacillus salivarius SD-5208→Increased Relative Weight of Immune Organs
The results showed that *Lactobacillus salivarius* supplementation [...] increased relative weights of the immune organs.
Effect
Beneficial
Effect size
Moderate
Lactobacillus salivarius SD-5208→Reduced Organ Injury from Escherichia coli
In addition, Lactobacillus salivarius supplementation alleviated organ injury caused by Escherichia coli O78 challenge.
Effect
Beneficial
Effect size
Large
Lactobacillus salivarius SD-5208→Reduced Organ Injury from Heat Stress
Lactobacillus salivarius supplementation alleviated organ injury caused by ... heat stress.
Effect
Beneficial
Effect size
Large
Lactobacillus salivarius UALb-07→Alleviated Organ Injury from Heat Stress
Lactobacillus salivarius supplementation alleviated organ injury caused by ... heat stress.
Effect
Beneficial
Effect size
Large
Lactobacillus salivarius UALb-07→Improved Growth Performance
The results showed that *Lactobacillus salivarius* supplementation improved growth performance, such as weight and longer shank length...
Effect
Beneficial
Effect size
Large
Lactobacillus salivarius UALb-07→Improved Immune Response
Lactobacillus salivarius results in enhanced immune response after IBDV vaccine immunization, enhanced specific antibody and IFN-γ production, and lymphocyte proliferation.
Effect
Beneficial
Effect size
Large
Lactobacillus salivarius UALb-07→Increased Relative Weight of Immune Organs
The results showed that *Lactobacillus salivarius* supplementation [...] increased relative weights of the immune organs.
Effect
Beneficial
Effect size
Moderate
Lactobacillus salivarius UALb-07→Reduced Odor-Causing Compound Concentrations
Lactobacillus salivarius supplementation ... decreased concentrations of odor-causing compounds.
Effect
Beneficial
Effect size
Moderate
Lactobacillus salivarius UALb-07→Reduced Organ Injury from Escherichia coli
In addition, Lactobacillus salivarius supplementation alleviated organ injury caused by Escherichia coli O78 challenge.
Effect
Beneficial
Effect size
Large
Lactobacillus salivarius UALs07→Alleviated Organ Injury
*Lactobacillus salivarius* supplementation alleviated organ injury caused by *Escherichia coli* O78 challenge and heat stress.
Effect
Beneficial
Effect size
Large
Lactobacillus salivarius UALs07→Improved Growth Performance
The results showed that *Lactobacillus salivarius* supplementation improved growth performance, such as weight and longer shank length...
Effect
Beneficial
Effect size
Large
Lactobacillus salivarius UALs07→Improved Immune Organ Development
Lactobacillus salivarius supplementation ... increased relative weights of the immune organs.
Effect
Beneficial
Effect size
Moderate
Lactobacillus salivarius UALs07→Improved Immune Response
Lactobacillus salivarius results in enhanced immune response after IBDV vaccine immunization, enhanced specific antibody and IFN-γ production, and lymphocyte proliferation.
Effect
Beneficial
Effect size
Large
Lactobacillus salivarius UALs07→Increased Organ Weights
Lactobacillus salivarius supplementation...increased relative weights of the immune organs.
Effect
Beneficial
Effect size
Moderate
Lactobacillus salivarius UALs07→Reduced Odor-Causing Compound Concentrations
Lactobacillus salivarius supplementation ... decreased concentrations of odor-causing compounds.
Effect
Beneficial
Effect size
Moderate
Lactobacillus salivarius UALs07→Reduced Organ Injury from Escherichia coli
In addition, Lactobacillus salivarius supplementation alleviated organ injury caused by Escherichia coli O78 challenge.
Effect
Beneficial
Effect size
Large
Lactobacillus salivarius UALs07→Reduced Organ Injury from Heat Stress
Lactobacillus salivarius supplementation alleviated organ injury caused by ... heat stress.
Effect
Beneficial
Effect size
Large