Assessment of the probiotic potential of a dairy product fermented by Propionibacterium freudenreichii in piglets.
- 2012-08-03
- Journal of Agricultural and Food Chemistry 60(32)
- Fabien J. Cousin
- B. Foligné
- S. Deutsch
- S. Massart
- S. Parayre
- Y. Le Loir
- G. Boudry
- G. Jan
- PubMed: 22823107
- DOI: 10.1021/jf302245m
Abstract
Dairy propionibacteria, including Propionibacterium freudenreichii , display promising probiotic properties, including immunomodulation. These properties are highly strain-dependent and rarely studied in a fermented dairy product. We screened 10 strains, grown in a newly developed fermented milk ultrafiltrate, for immunomodulatory properties in vitro. The most anti-inflammatory strain, P. freudenreichii BIA129, was further tested on piglets. P. freudenreichii -fermented product improved food intake and growth of piglets. Colonic mucosa explants of treated pigs secreted less interleukin 8 (-25%, P < 0.05) and tumor necrosis factor α (-20%, P < 0.05), either in basal conditions or after a lipopolysaccharide challenge. By contrast, the gut structure, barrier function (measured ex vivo in Ussing chambers), microbial diversity (assessed by 16S rRNA pyrosequencing), and colonic short-chain fatty acid content were unchanged, assuming maintenance of normal intestinal physiology. In conclusion, this work confirms in vivo probiotic properties of dairy propionibacteria-fermented products, which are promising for the prevention or healing of inflammatory bowel diseases.
Research Insights
Supplement | Health Outcome | Effect Type | Effect Size |
---|---|---|---|
Propionibacterium freudenreichii MAK10P23F | Maintenance of Normal Intestinal Function | Neutral | Small |
Propionibacterium freudenreichii MAK10P23F | Reduced Interleukin-8 Secretion | Beneficial | Moderate |
Propionibacterium freudenreichii MAK10P23F | Reduced Tumor Necrosis Factor Alpha | Beneficial | Moderate |