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Bacillus subtilis HU58 and Bacillus coagulans SC208 Probiotics Reduced the Effects of Antibiotic-Induced Gut Microbiome Dysbiosis in an M-SHIME® Model

  • 2020-07-11
  • Microorganisms 8(7)
    • M. Marzorati
    • P. Abbeele
    • Sarah S Bubeck
    • Thomas Bayne
    • K. Krishnan
    • Aicacia Young
    • D. Mehta
    • A. DeSouza


Benefits associated with probiotic use have been reported; however, the mechanisms behind these benefits are poorly understood. The effects of a probiotic formulation (MegaDuo™) containing Bacillus coagulans SC208 and Bacillus subtilis HU58 on intestinal permeability and immune markers was assessed using a combination of the in vitro gut model, the mucosal simulator of the human intestinal microbial ecosystem (M-SHIME®), and an in vitro inflammatory bowel disease-like Caco-2/THP1 co-culture model in both healthy and antibiotic-induced dysbiosis conditions. Established M-SHIME® proximal colon vessels were treated with/without clindamycin (1 week) and then with/without daily MegaDuo™ treatment (2 weeks). The mucosal and luminal microbial communities were sampled weekly. Suspensions were removed from the proximal colon vessels after 1 and 2 weeks of MegaDuo™ treatment and added to the co-culture system. Transepithelial resistance (membrane barrier function), cytokine/chemokine release, and NFκB activity were then measured. Under conditions of antibiotic-induced dysbiosis, suspensions from MegaDuo™ treated vessels showed reduced gut membrane barrier damage and decreased levels of TNFα and IL-6 compared with suspensions from untreated vessels; no appreciable differences were observed under healthy conditions. MegaDuo™ treatment had no effect on NFκB activity of THP1-Blue™ cells. The potential benefits of MegaDuo™ treatment appeared most evident after 2 weeks of treatment.

Keywords: M-SHIME; dysbiosis; gut barrier; gut inflammation; gut microbiome; immunomodulation; probiotics.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Bacillus coagulans SC208Reduced Gut Membrane Barrier DamageBeneficial
Bacillus subtilis HU58Reduced Gut Barrier DefectBeneficial
Bacillus subtilis HU58Reduced Interleukin-6 LevelsBeneficial
Bacillus subtilis HU58Reduced TNF-α LevelsBeneficial

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