Skip to main content

Bifidobacterium infantis attenuates colitis by regulating T cell subset responses.

  • 2014
  • World Journal of Gastroenterology 20(48)
    • L. Zuo
    • Kai-Tao Yuan
    • Li Yu
    • Qing-Hong Meng
    • P. C. Chung
    • Ding-hua Yang


Aim: to investigate the effect of Bifidobacterium infantis (B. infantis) on the T cell subsets and in attenuating the severity of experimental colitis in mice.

Methods: Normal BALB/c mice were fed different doses of B. infantis for 3 wk, and T cell subsets and related cytokine profiles in mesenteric lymph nodes (MLNs) were detected by flow cytometry and real-time RT-PCR. Colitis was induced by administration of trinitrobenzene sulfonic acid (TNBS) in mice. Before colitis induction, mice were fed high dose B. infantis for 3 wk. Cytokine profiles in MLNs and histological changes of colonic tissue were examined 6 d after colitis induction.

Results: No significant change in cytokine profiles was observed in normal mice fed low dose B. infantis. However, Th1-related cytokines (IL-2, IFN-γ, IL-12p40), Th17-related transcription factor and cytokines (RORγt, IL-21, IL-23), regulatory T cell (Treg)-related transcription factor and cytokines (Foxp3, IL-10) were increased in normal mice fed high dose B. infantis. Furthermore, flow cytometry assay showed B. infantis increased the numbers of CD4(+)Foxp3(+) Tregs and Th17 cells in MLNs. Colitis was successfully induced by TNBS in mice, characterized by colonic inflammation and aberrant Th1 and Th17 responses. Feeding high dose B. infantis for 3 wk before colitis induction decreased the inflammatory cell infiltration and goblet cell depletion and restored the intestinal epithelium. In addition, B. infantis feeding reduced Th1-related cytokines (T-bet, IL-2 and IFN-γ) and Th17-related cytokines (IL-12p40, RORγt, IL-17A, IL-21 and IL-23), and increased Treg-related molecules (Foxp3, IL-10 and TGF-β) in colitis mice.

Conclusion: B. infantis effectively attenuates TNBS-induced colitis by decreasing Th1 and Th17 responses and increasing Foxp3(+) Treg response in the colonic mucosa.

Keywords: Bifidobacterium; Colitis; Cytokines; Regulatory T cells; Th17.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Bifidobacterium infantisIncreased Regulatory T-CellsBeneficial
Bifidobacterium infantisReduced Immune Cell InfiltrationBeneficial
Bifidobacterium infantis MAK22B04IReduced Inflammatory CytokinesBeneficial
Bifidobacterium infantis MAK22B04IReduced Severity of Acute ColitisBeneficial
⬆ Back to top
Supplement Research and Comparison Website: evidence-based information about supplements, their benefits, potential risks, and their efficacy.
Receive updates about our products, services, sales, and special offers. Unsubscribe anytime. See our Privacy Policy for details on how we handle your information.

Join Our Community

Use to get in touch.