Skip to main content

Cellular injuries of spray-dried Lactobacillus spp. isolated from kefir and their impact on probiotic properties.

  • 2011-01
  • International Journal of Food Microbiology 144(3)
    • M. Golowczyc
    • Joana G. L. Silva
    • P. Teixeira
    • G. D. De Antoni
    • A. Abraham


The injuries caused by spray drying (SD) of three potential probiotic lactobacilli isolated from kefir grains and the impact on some probiotic properties, were evaluated. Results demonstrated that Lactobacillus plantarum 83114 and L. kefir 8321 showed a slight reduction of viability (0.11 and 0.29 log CFU/ml respectively) after SD process, and L. kefir 8348 was found to be more sensitive to the process with a reduction in viability of 0.70 log CFU/ml. Neither membrane damage, evaluated by increased sensitivity to NaCl, lysozyme, bile salt and penicillin G, nor changes in acidifying activity in MRS and milk by lactobacilli were detected after SD. L. plantarum 83114 and L. kefir 8321 after SD did not lose their capacity to adhere to intestinal cells. Nevertheless, L. kefir 8348 showed a significant loss of adhesion capacity after SD. In addition, rehydrated spray-dried L. kefir 8321 retained the ability to protect against Salmonella invasion of intestinal cells. This effect was observed when L. kefir is co-incubated with Salmonella before invasion assay. This work shows that the membrane integrity evaluated by indirect methods and some probiotic properties of lactobacilli isolated from kefir did not change significantly after SD, and these powders could be used in functional foods applications.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus kefirMaintained Cellular Viability Post Spray DryingBeneficial
Lactobacillus kefirPreserved Membrane IntegrityBeneficial
Lactobacillus kefirReduced Intestinal Cell AdhesionBeneficial
Lactobacillus kefirReduced Salmonella IncidenceBeneficial
Lactobacillus kefiriReduced Adhesion Capacity (L. kefir 8348)Harmful
Lactobacillus kefiriReduced Intestinal Cell AdhesionBeneficial
Lactobacillus kefiriReduced Salmonella IncidenceBeneficial

Pillser helps you make informed health decisions by providing comprehensive, unbiased information about supplements. This includes detailed research on supplement ingredients, their benefits, potential risks, and their overall efficacy. You can contribute by sharing your feedback and suggestions.

Send us an email at