Colonic goblet cell-associated antigen passages mediate physiologic and beneficial translocation of live gut bacteria in preweaning mice.
- 2025-04-01
- Nature microbiology 10(4)
- Sreeram Udayan
- Alexandria N Floyd
- Vini John
- Bibiana E Barrios
- Brigida A Rusconi
- Keely G McDonald
- Ellen Merrick Schill
- Devesha H Kulkarni
- Andrew L Martin
- Rafael Gutierrez
- Khushi B Talati
- Dalia L Harris
- Sushma Sundas
- Kayla M Burgess
- Jocelyn T Pauta
- Elisabeth L Joyce
- Jacqueline D Wang
- Leslie D Wilson
- Kathryn A Knoop
- Phillip I Tarr
- Chyi-Song Hsieh
- Rodney D Newberry
- PubMed: 40169738
- DOI: 10.1038/s41564-025-01965-1
Study Design
- Population
- preweaning (day of life 17), but not adult (day of life 35), mice
- Methods
- using bacterial culture techniques, we show that a subset of live gut-resident bacteria translocate and disseminate to extraintestinal tissues (mesenteric lymph nodes and spleen)
- Animal Study
Gut-resident microorganisms have time-limited effects in distant tissues during early life. However, the reasons behind this phenomenon are largely unknown. Here, using bacterial culture techniques, we show that a subset of live gut-resident bacteria translocate and disseminate to extraintestinal tissues (mesenteric lymph nodes and spleen) in preweaning (day of life 17), but not adult (day of life 35), mice. Translocation and dissemination in preweaning mice appeared physiologic as it did not induce an inflammatory response and required host goblet cells, the formation of goblet cell-associated antigen passages, sphingosine-1-phosphate receptor-dependent leukocyte trafficking and phagocytic cells. One translocating strain, Lactobacillus animalisWU, showed antimicrobial activity against the late-onset sepsis pathogen Escherichia coli ST69 in vitro, and its translocation was associated with protection from systemic sepsis in vivo. While limited in context, these findings challenge the idea that translocation of gut microbiota is pathological and show physiologic and beneficial translocation during early life.
Research Insights
| Supplement | Dose | Health Outcome | Effect Type | Effect Size | Source |
|---|---|---|---|---|---|
| Lactobacillus salivarius UCC118 | — | Reduced Risk of Sepsis | Beneficial | Moderate | View sourceits translocation was associated with protection from systemic sepsis in vivo |
| Lactobacillus salivarius UCC118 | — | Reduced Sepsis | Beneficial | Moderate | View sourceits translocation was associated with protection from systemic sepsis in vivo. |