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Colonization and Immunomodulation by Lactobacillus reuteri ATCC 55730 in the Human Gastrointestinal Tract

  • 2004-02
  • Applied and Environmental Microbiology 70(2)
    • N. Valeur
    • P. Engel
    • N. Carbajal
    • E. Connolly
    • K. Ladefoged

Abstract

Lactobacillus reuteri ATCC 55730 is a probiotic (health-promoting) bacterium widely used as a dietary supplement. This study was designed to examine local colonization of the human gastrointestinal mucosa after dietary supplementation with L. reuteri ATCC 55730 and to determine subsequent immune responses at the colonized sites. In this open clinical investigation, 10 healthy volunteers and 9 volunteers with ileostomy underwent gastroscopy or ileoscopy and biopsy samples were taken from the stomach, duodenum, or ileum before and after supplementation with 4 x 10(8) CFU of live L. reuteri ATCC 55730 lactobacilli per day for 28 days. Biopsy specimen colonization was analyzed using fluorescence in situ hybridization with a molecular beacon probe, and immune cell populations were determined by immunostaining. Endogenous L. reuteri was detected in the stomach of 1 subject and the duodenum of 3 subjects (out of 10 subjects). After L. reuteri ATCC 55730 supplementation, the stomachs of 8 and the duodenums of all 10 subjects were colonized. Three ileostomy subjects (of six tested) had endogenous L. reuteri at baseline, while all six displayed colonization after L. reuteri supplementation. Gastric mucosal histiocyte numbers were reduced and duodenal B-lymphocyte numbers were increased by L. reuteri ATCC 55730 administration. Furthermore, L. reuteri administration induced a significantly higher amount of CD4-positive T-lymphocytes in the ileal epithelium. Dietary supplementation with the probiotic L. reuteri ATCC 55730 induces significant colonization of the stomach, duodenum, and ileum of healthy humans, and this is associated with significant alterations of the immune response in the gastrointestinal mucosa. These responses may be key components of a mechanism by which L. reuteri ATCC 55730 exerts its well-documented probiotic effects in humans.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus reuteriIncreased CD4-Positive T-Lymphocytes in Ileal EpitheliumBeneficial
Large
Lactobacillus reuteriIncreased Duodenal B-Lymphocyte CountsBeneficial
Moderate
Lactobacillus reuteriIncreased Gastrointestinal ColonizationBeneficial
Large
Lactobacillus reuteriReduced Gastric Mucosal HistiocytesBeneficial
Moderate
Lactobacillus reuteri HA-188Increased CD4-Positive T-LymphocytesBeneficial
Large
Lactobacillus reuteri HA-188Increased Duodenal B-Lymphocyte CountsBeneficial
Moderate
Lactobacillus reuteri HA-188Increased Probiotic Bacteria ColonizationBeneficial
Large
Lactobacillus reuteri HA-188Reduced Gastric Mucosal Histiocyte NumbersBeneficial
Moderate
Lactobacillus reuteri LRE02Increased CD4-Positive T-Lymphocytes in Ileal EpitheliumBeneficial
Large
Lactobacillus reuteri LRE02Increased Duodenal B-Lymphocyte CountsBeneficial
Moderate
Lactobacillus reuteri LRE02Increased Gastrointestinal Tract ColonizationBeneficial
Large
Lactobacillus reuteri LRE02Reduced Gastric Mucosal Histiocyte NumbersBeneficial
Moderate
Lactobacillus reuteri MAK02L14RGastrointestinal Tract ColonizationBeneficial
Large
Lactobacillus reuteri MAK02L14RIncreased CD4-Positive T-Lymphocytes in Ileal EpitheliumBeneficial
Large
Lactobacillus reuteri MAK02L14RIncreased Duodenal B-Lymphocyte CountsBeneficial
Moderate
Lactobacillus reuteri MAK02L14RReduced Gastric Mucosal HistiocytesBeneficial
Moderate
Lactobacillus reuteri OsfortisIncreased CD4-Positive T-Lymphocytes in Ileal EpitheliumBeneficial
Large
Lactobacillus reuteri OsfortisIncreased Duodenal B-Lymphocyte CountsBeneficial
Moderate
Lactobacillus reuteri OsfortisReduced Gastric Mucosal Histiocyte NumbersBeneficial
Moderate
Lactobacillus reuteri SD-5865Gastrointestinal Tract ColonizationBeneficial
Large
Lactobacillus reuteri SD-5865Increased CD4-Positive T-Lymphocytes in Ileal EpitheliumBeneficial
Large
Lactobacillus reuteri SD-5865Increased Duodenal B-Lymphocyte CountsBeneficial
Moderate
Lactobacillus reuteri SD-5865Reduced Gastric Mucosal Histiocyte NumbersBeneficial
Moderate
Lactobacillus reuteri UALg-05Increased Duodenal B-Lymphocyte CountsBeneficial
Moderate
Lactobacillus reuteri UALg-05Reduced Gastric Mucosal Histiocyte NumbersBeneficial
Moderate
Lactobacillus reuteri UALre-16Gastrointestinal Tract Probiotic ColonizationBeneficial
Large
Lactobacillus reuteri UALre-16Increased Duodenal B-Lymphocyte CountsBeneficial
Moderate
Lactobacillus reuteri UALre-16Reduced Gastric Mucosal Histiocyte NumbersBeneficial
Moderate

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