Complete genome analysis of Lactobacillus fermentum YLF016 and its probiotic characteristics.
- 2022-01
- Microbial Pathogenesis 162
- Li-hong Zhang
- Hong-Bo Ma
- M. F. Kulyar
- Huachun Pan
- Kewei Li
- Aoyun Li
- Q. Mo
- Yaping Wang
- Hailong Dong
- Yuhua Bao
- Jia-kui Li
- PubMed: 34597776
- DOI: 10.1016/j.micpath.2021.105212
Abstract
Lactobacillus fermentum (L. fermentum) YLF016 is a well-characterized probiotic with several favorable characteristics. This study aimed to analyze the probiotic characteristics of L. fermentum and uncover the genes implicated in its potential probiotic ability on the base of its genomics features. The complete genome of L. fermentum YLF016 was found to have a circular chromosome of 2,094,354 bp, and 51.46% G + C content without any plasmid. Its chromosome contained 2,130 predicted protein-encoding genes, 58 tRNA, and 15 rRNA-encoding genes. Also, it was found to have many other probiotic properties, such as a high survival rate in the gastrointestinal tract with strong adherence to intestinal cells, antibacterial activity against pathogens, and antioxidant activity. Moreover, the genome sequence analysis demonstrated specific genes coding for carbon metabolism pathway, genetic adaption, stress resistance, and adhesive ability. Further analysis revealed its non-hemolytic activity and its non-functional ability of virulence factors. In conclusion, L. fermentum YLF016 possesses many valuable probiotic properties that refer to its potential probiotic ability.
Keywords: Complete genome; Genetic; Lactobacillus fermentum; Probiotics.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus fermentum LF61 | Absence of Hemolytic Activity | Beneficial | Moderate |
| Lactobacillus fermentum LF61 | Increased Antibacterial Activity Against Pathogens | Beneficial | Large |
| Lactobacillus fermentum LF61 | Increased Antioxidant Activity | Beneficial | Moderate |
| Lactobacillus fermentum LF61 | Increased Survival in Gastrointestinal Tract | Beneficial | Large |
| Lactobacillus fermentum LF61 | Reduced Virulence Factors | Beneficial | Moderate |
| Lactobacillus fermentum UALf-32 | Absence of Hemolytic Activity | Beneficial | Moderate |
| Lactobacillus fermentum UALf-32 | Enhanced Intestinal Epithelial Cell Adhesion | Beneficial | Large |
| Lactobacillus fermentum UALf-32 | Increased Antibacterial Activity Against Pathogens | Beneficial | Large |
| Lactobacillus fermentum UALf-32 | Increased Antioxidant Activity | Beneficial | Moderate |
| Lactobacillus fermentum UALf-32 | Increased Gastric Survival Rate | Beneficial | Large |
| Lactobacillus fermentum UALf-32 | Reduced Virulence Factors | Beneficial | Moderate |