Construction of oxidized dextran-based pH/ROS responsive hydrogel with live bacterial microcapsules for accelerating diabetic wound healing via reducing inflammatory factors.
- 2026-04
- International journal of biological macromolecules 354
- PubMed: 41831510
- DOI: 10.1016/j.ijbiomac.2026.151405
Study Design
- Methods
- Living bacteria-loaded oxidized dextran hydrogel fabricated with Lactobacillus bulgaricus encapsulated in gelatin microcapsules via microcapsule technology, and mangiferin incorporated via dynamic borate ester bond; pH/ROS responsive hydrogel (OPM@GLB) prepared through Schiff base reaction; tested in vitro and in vivo.
Diabetic wound (DW) is characterized by chronic inflammation and bacterial infection, making its healing a continuing clinical challenge. Herein, a living bacteria-loaded oxidized dextran hydrogel is successfully fabricated to accelerate wound healing via regulating inflammatory factors. Lactobacillus bulgaricus (LB), as a live microorganism, was encapsulated in the hydrogel via microcapsule technology with gelatin as a substance to reduce secondary infections and ensure the survival of LB. Gelatin microcapsules and oxidized dextran were used to prepare a pH/ROS responsive hydrogel (OPM@GLB) through a Schiff base reaction. Furthermore, mangiferin was also incorporated into the hydrogel by a dynamic borate ester bond to support desirable nutrition for LB. In vitro and in vivo results showed that the OPM@GLB hydrogel exhibited significant antibacterial and anti-inflammatory properties and promoted angiogenesis and collagen deposition to accelerate diabetic wound healing. This study may offer new insights for treating diabetic wound in the clinic.
Research Insights
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