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Dietary Lactobacillus plantarum ST-III alleviates the toxic effects of triclosan on zebrafish (Danio rerio) via gut microbiota modulation.

  • 2019-01
  • Fish & Shellfish Immunology 84
    • Luxiu Zang
    • Yan Ma
    • Wenhao Huang
    • Yuhang Ling
    • Limei Sun
    • Xuedong Wang
    • Aibing Zeng
    • R. Dahlgren
    • Caihong Wang
    • Huili Wang

Abstract

The probiotics, Lactobacillus plantarum ST-III, plays an important role in modulating microbiota and alleviating intestinal metabolic disorders. Herein, we reported that Lactobacillus increases biodiversity of zebrafish gut flora, and attenuates toxic effects from chronic triclosan (TCS) exposure. Lactobacillus-feeding recovered the species and amount of microorganisms in the intestines of zebrafish, and inhibited toxin production by saprophytic bacterial growth. Abnormal physiological indexes and malonaldeyhde content resulting from TCS exposure were effectively alleviated. Additionally, lipid-metabolism disorders, such as increased triglyceride and total cholesterol levels, were attenuated by a probiotics diet. The number of CD4+ T cell lymphocytes in the lamina propria of the duodenal mucosa was decreased in zebrafish receiving a Lactobacillus diet compared to the TCS-exposed group, showing a consistent expression trend for six immune genes (NF-κB, IL-1β, TNF-α, lysozyme, TLR4α, IL-10) in the intestinal mucosa. Histopathological observations of intestines, spleen and kidney showed that TCS exposure produced severe damage to the morphology and structure of immune and metabolism-related organs. Lactobacillus was capable of mitigating this damage, but bile salt hydrolase, an active extract of Lactobacillus, was not an effective mitigation strategy. The Lactobacillus-induced decrease in the number of inflammatory cells confirmed its role in preventing inflammatory injury. Three behavioral tests (T-maze, bottom dwelling and social interaction) indicated that a probiotics diet improved zebrafish movement and learning/memory capacity, effectively alleviating anxiety behavior due to TCS exposure. These findings inform development of beneficial strategies to alleviate intestinal metabolic syndromes and neurodegenerative diseases resulting from exposure to environmental contaminants through modifying gut flora with a probiotics diet.

Keywords: Attenuated abnormal behavior; Inflammation; Lactobacillus plantarum ST-III; Lipid metabolism; Metabolic disorder; Triclosan.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus plantarumAlleviated Intestinal Metabolic DisordersBeneficial
Large
Lactobacillus plantarumEnhanced Gut Microbiota DiversityBeneficial
Moderate
Lactobacillus plantarumReduced InflammationBeneficial
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Lactobacillus plantarumReduced Malonaldehyde LevelsBeneficial
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Lactobacillus plantarumReduced Pro-Inflammatory CellsBeneficial
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Lactobacillus plantarumRestored Lipid MetabolismBeneficial
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Lactobacillus plantarum VPro 10Enhanced Gut Microbiota DiversityBeneficial
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Lactobacillus plantarum VPro 10Improved Blood Lipid MetabolismBeneficial
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Lactobacillus plantarum VPro 10Reduced Bacterial Toxin ProductionBeneficial
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Lactobacillus plantarum VPro 10Reduced CD4+ T Cell Lymphocytes in Intestinal MucosaBeneficial
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Lactobacillus plantarum VPro 10Reduced Immune Gene ExpressionBeneficial
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Lactobacillus sakei proBio65Improved Behavioral HealthBeneficial
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Lactobacillus sakei proBio65Improved Blood Lipid MetabolismBeneficial
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Lactobacillus sakei proBio65Improved Gut Microbiota DiversityBeneficial
Moderate
Lactobacillus sakei proBio65Reduced Inflammation LevelsBeneficial
Large
Lactobacillus sakei proBio65Reduced ToxicityBeneficial
Large

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