Dose-Response Recovery of Probiotic Strains in Simulated Gastro-Intestinal Passage.

2020-01-13
Microorganisms 8(1)
- Sofia Forssten
- Arthur C Ouwehand

PubMed: 31941108
DOI: 10.3390/microorganisms8010112

Study Design

Methods: Two different doses, 7 × 10^9 colony forming units (CFU) and 7 × 10^10 CFU, of a probiotic mixture (Bifidobacterium lactis Bl-04, Lactobacillus acidophilus La-14, Lactobacillus paracasei Lpc-37, and Lactobacillus plantarum Lp-115) or placebo were tested. The four strains were quantified by qPCR and the metabolites analyzed by gas chromatography.

The probiotic definition stipulates "adequate amounts". Here, we investigated the metabolic output and recovery rate of probiotic strains using a simulated upper gastro-intestinal passage and colonic fermentation. Two different doses, 7 × 10⁹ colony forming units (CFU) and 7 × 10¹⁰ CFU, of a probiotic mixture (Bifidobacterium lactis Bl-04, Lactobacillus acidophilus La-14, Lactobacillus paracasei Lpc-37, and Lactobacillus plantarum Lp-115) or placebo were tested. The four strains were quantified by qPCR and the metabolites analyzed by gas chromatography. There was a dose-response in the detection of all four strains. There was a slightly larger increase between the two doses for L. paracasei Lpc-37 as compared with the other strains; this may suggest a greater robustness of this strain. Compared with the placebo, the high dose simulations generated more propionic acid and a higher total of short chain fatty acids (SCFA). Higher doses of a species are required to reach measurable increases above the baseline level of this species.

Research Insights

Supplement	Dose	Health Outcome	Effect Type	Effect Size	Source
Bifidobacterium lactis SD-5219	—	Improved Gastrointestinal Recovery	Beneficial	Small	View source There was a dose-response in the detection of all four strains. There was a slightly larger increase between the two doses for L. paracasei Lpc-37 as compared with the other strains; this may suggest a greater robustness of this strain.
Bifidobacterium lactis SD-5219	—	Increased Short-Chain Fatty Acid Production	Beneficial	Small	View source Compared with the placebo, the high dose simulations generated more propionic acid and a higher total of short chain fatty acids (SCFA).
Lactobacillus acidophilus SD-5212	—	Increased Short-Chain Fatty Acid Production	Beneficial	Small	View source Compared with the placebo, the high dose simulations generated more propionic acid and a higher total of short chain fatty acids (SCFA).
Lactobacillus acidophilus SD-5212	—	Viable Probiotic Strain Recovery	Beneficial	Small	View source There was a dose-response in the detection of all four strains. There was a slightly larger increase between the two doses for L. paracasei Lpc-37 as compared with the other strains; this may suggest a greater robustness of this strain.