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Dual function of Lactobacillus kefiri DH5 in preventing high-fat-diet-induced obesity: direct reduction of cholesterol and upregulation of PPAR-α in adipose tissue.

  • 2017-08-29
  • Molecular Nutrition & Food Research 61(11)
    • Dong‐Hyeon Kim
    • Dana Jeong
    • Il‐Byeong Kang
    • Hyunsook Kim
    • K. Song
    • K. Seo

Abstract

Scope: Kefir consumption inhibits the development of obesity and non-alcoholic fatty liver disease (NALFD) in mice fed 60% high-fat diet (HFD). To identify the key contributor of this effect, we isolated lactic acid bacteria (LAB) from kefir and examined their anti-obesity properties from in vitro screening and in vivo validation.

Methods and results: Thirteen kefir LAB isolates were subjected to survivability test using artificial gastrointestinal environment and cholesterol-reducing assay. Lactobacillus kefiri DH5 showed 100% survivability in gastrointestinal environments and reduced 51.6% of cholesterol; thus, this strain was selected for in vivo experiment. Compared to the HFD-saline group, the HFD-DH5 group showed significantly lower body weight (34.68 versus 31.10 g; p < 0.001), epididymal adipose tissue weight (1.39 versus 1.05 g; p < 0.001), blood triglyceride (38.2 versus 31.0 mg/dL; p < 0.01) and LDL-cholesterol levels (19.4 versus 15.7 mg/dL; p < 0.01). In addition, L. kefiri DH5 administration significantly modulated gut microbiota of HFD-fed mice. The hepatic steatosis was significantly milder (Lesion score, 2.1 versus 1.2; p < 0.001) and adipocyte diameter was significantly smaller (65.1 versus 42.2 μm; p < 0.001) in the HFD-DH5 group. L. kefiri DH5 upregulated PPAR-α, FABP4, and CPT1 expression in the epididymal adipose tissues (2.29-, 1.77-, and 2.05-fold change, respectively), suggesting a reduction in adiposity by stimulating fatty acid oxidation.

Conclusion: L. kefiri DH5 exerts anti-obesity effects by direct reduction of cholesterol in the lumen and upregulation of PPAR-α gene in adipose tissues.

Keywords: Cholesterol reduction; High fat diet; Lactobacillus kefiri; Obesity; PPAR-α.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus kefirIncreased CPT1 ExpressionBeneficial
Moderate
Lactobacillus kefirIncreased FABP4 ExpressionBeneficial
Moderate
Lactobacillus kefirIncreased PPAR-Alpha ExpressionBeneficial
Moderate
Lactobacillus kefirReduced Adipocyte DiameterBeneficial
Large
Lactobacillus kefirReduced Adipose Tissue MassBeneficial
Large
Lactobacillus kefirReduced Blood Triglyceride LevelsBeneficial
Large
Lactobacillus kefirReduced Body WeightBeneficial
Large
Lactobacillus kefirReduced Hepatic SteatosisBeneficial
Moderate
Lactobacillus kefirReduced LDL CholesterolBeneficial
Large
Lactobacillus kefiriAltered Gut MicrobiotaBeneficial
Moderate
Lactobacillus kefiriReduced Blood Triglyceride LevelsBeneficial
Large
Lactobacillus kefiriReduced Body WeightBeneficial
Large
Lactobacillus kefiriReduced Epididymal Fat MassBeneficial
Large
Lactobacillus kefiriReduced Hepatic SteatosisBeneficial
Moderate
Lactobacillus kefiriReduced LDL CholesterolBeneficial
Large

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