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Study Design

Methods
UHPLC-DAD-MSn analysis of dehulled mung bean cotyledons during short-term germination (4-48 h)
Duration
4-48 h
Funding
Unclear
Phenolic compounds in mung bean (Vigna radiata L. Wilczek) are important bioactive constituents whose levels and composition change during germination. However, their distribution between seed fractions (cotyledon and hull) and their compositional changes in dehulled cotyledons during early germination remain insufficiently understood. This study investigated how dehulling affects phenolic compound distribution and examined how short-term germination (4-48 h) influences the phenolic content and composition of dehulled mung bean sprouts using UHPLC-DAD-MSn. Among the 12 detected phenolic compounds, 11 were tentatively identified. Total phenolic content was highest in the hull (10,055.3 μg/g dw) and lowest in the ungerminated cotyledon (304.3 μg/g dw). Vitexin and isovitexin were the predominant flavonoids in both fractions, with the hull contributing 66.0% and 75.5% of total vitexin and isovitexin, respectively. During germination, several apigenin glycosides, particularly isovitexin-O-rhamnoside, and acylated apigenin derivatives, accumulated. These findings highlight time-dependent changes in flavonoid composition during early mung bean germination.

Research Insights

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