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Study Design

Population
mice (4 groups of 6)
Methods
Phytochemical screening, antioxidant assays, and antidepressant tests (forced swim, tail suspension, elevated plus maze) in four groups: negative control, imipramine (100 mg/kg), barley extract (400 mg/kg), wheat extract (400 mg/kg)
Funding
Unclear

Background

The consumption of green juice of Barley and wheat grass is widely increases because of its therapeutic benefits.

Objective

The study aimed to investigate phytochemicals and evaluate the antioxidant and antidepressant activity of aqueous extract of barley and wheat grass.

Material and methods

This study included phytochemical screening, evaluation of antioxidant and antidepressant activities. Four groups consisting of six mice in each group. Negative control with stressed induced mice; imipramine group (100 mg/kg), barley and wheat extract group (400 mg/kg) respectively. Forced swim, tail suspension and elevated plus maize test were carried out to evaluate the antianxiety and antidepressant activity.

Results

Phytochemical screening of barley and wheat grass extract showed secondary metabolites such as alkaloids, Saponin, Tannins, Phenolic, Carbohydrate, Glycosides, Flavonoids, and Proteins. The mean total phenolic content of aqueous extract of barley and wheat grass was 160.996 ± 0.656, 135.63 ± 1.184 mg equivalent of GAE/g respectively. The total flavonoid content of aqueous extract of barley and wheat grass was 153.42 ± 0.40, 133.14 ± 0.43 mg equivalent of quercetin/g respectively. The extracts were proved to be an effective radical scavenger in all antioxidant assays. Forced swim and tail suspension test showed a significant (∗p < 0.05 and ∗∗p < 0.01) decrease in an immobility time. In elevated plus maize, there was a significant (∗∗p < 0.01) increase in average time spent on the open arm of the extract-treated group as compared to the negative control.

Conclusion

It shows that barley and wheat grass extract has antidepressant effect.

Research Insights

SupplementDoseHealth OutcomeEffect TypeEffect SizeSource
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