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Abstract

The effect of dietary inclusion of Bacillus subtilis DSM 32315 on the intestinal health and growth performance of Cobb 500 male broilers subjected to a Clostridium perfringens-induced necrotic enteritis (NE) challenge was determined in 2 experiments. In experiment 1, chicks were randomly assigned to 4 treatments of 10 replicate/treatment. In experiment 2, chicks were randomly assigned to 4 treatments of 12 replicates/treatment. The experimental treatments were non-infected, non-supplemented control, infected, non-supplemented control (IC), infected + Bacillus subtilis DSM 32315 (B. subtilis DSM 32315), infected + bacitracin methylene disalicylate (BMD). In both experiments, NE was induced by oral inoculation of toxin producing C. perfringens on 3 consecutive days between 17 and 20 D of age, following exposure of birds to pre-disposing conditions. At day 28 (experiment 1), broilers fed diets with B. subtilis DSM 32315 exhibited a significantly higher body weight, lower mortality, and intestinal NE lesion score, compared to the IC treatment. At day 42 (experiment 2), B. subtilis DSM 32315 supplementation significantly improved BW, feed conversion ratio, production efficiency factor, NE lesion score, and mortality, compared to IC treatment. The effect of B. subtilis DSM 32315 on intestinal integrity of NE challenged chickens was evaluated with histomorphometry. A significantly shallower crypt depth and higher villus height to crypt depth ratio were observed in the mid-intestine of birds belonging to the B. subtilis DSM 32315 group, compared to the IC group. Furthermore, B. subtilis DSM 32315 supplementation significantly reduced the enteritis index associated with NE. In both experiments, the effect of B. subtilis DSM 32315 on the phenotypic measurements of NE and performance was comparable to the effect observed with BMD supplementation. In conclusion, supplementation of the direct fed microbial strain B. subtilis DSM 32315 can ameliorate the pathology and performance detriments associated with NE.

Keywords: Bacillus subtilis; Clostridium perfringens; broiler; necrotic enteritis; performance.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Bacillus subtilis AB22Improved Body WeightBeneficial
Large
Bacillus subtilis AB22Improved Feed Conversion RatioBeneficial
Large
Bacillus subtilis AB22Improved Production EfficiencyBeneficial
Large
Bacillus subtilis AB22Increased Villus Height to Crypt Depth RatioBeneficial
Moderate
Bacillus subtilis AB22Reduced Colitis-Related MortalityBeneficial
Large
Bacillus subtilis AB22Reduced Crypt DepthBeneficial
Moderate
Bacillus subtilis AB22Reduced Enteritis IndexBeneficial
Large
Bacillus subtilis AB22Reduced Intestinal NE Lesion ScoreBeneficial
Large
Bacillus subtilis SNZ-1972Improved Body WeightBeneficial
Large
Bacillus subtilis SNZ-1972Improved Feed Conversion RatioBeneficial
Large
Bacillus subtilis SNZ-1972Increased Villus Height to Crypt Depth RatioBeneficial
Moderate
Bacillus subtilis SNZ-1972Reduced Colitis-Related MortalityBeneficial
Large
Bacillus subtilis SNZ-1972Reduced Crypt DepthBeneficial
Moderate
Bacillus subtilis SNZ-1972Reduced Enteritis IndexBeneficial
Large
Bacillus subtilis SNZ-1972Reduced Intestinal NE Lesion ScoreBeneficial
Large

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