Effect of Bifidobacterium breve on the Intestinal Microbiota of Coeliac Children on a Gluten Free Diet: A Pilot Study
- 2016-10-22
- Nutrients 8(10)
- Andrea Quagliariello
- I. Aloisio
- Nicole Bozzi Cionci
- D. Luiselli
- G. D’Auria
- L. Martinez-Priego
- David Pérez-Villarroya
- T. Langerholc
- Maša Primec
- D. Mičetić-Turk
- D. Di Gioia
- PubMed: 27782071
- DOI: 10.3390/NU8100660
Abstract
Coeliac disease (CD) is associated with alterations of the intestinal microbiota. Although several Bifidobacterium strains showed anti-inflammatory activity and prevention of toxic gliadin peptides generation in vitro, few data are available on their efficacy when administered to CD subjects. This study evaluated the effect of administration for three months of a food supplement based on two Bifidobacterium breve strains (B632 and BR03) to restore the gut microbial balance in coeliac children on a gluten free diet (GFD). Microbial DNA was extracted from faeces of 40 coeliac children before and after probiotic or placebo administration and 16 healthy children (Control group). Sequencing of the amplified V3-V4 hypervariable region of 16S rRNA gene as well as qPCR of Bidobacterium spp., Lactobacillus spp., Bacteroides fragilis group Clostridium__sensu stricto and enterobacteria were performed. The comparison between CD subjects and Control group revealed an alteration in the intestinal microbial composition of coeliacs mainly characterized by a reduction of the Firmicutes/Bacteroidetes ratio, of Actinobacteria and Euryarchaeota. Regarding the effects of the probiotic, an increase of Actinobacteria was found as well as a re-establishment of the physiological Firmicutes/Bacteroidetes ratio. Therefore, a three-month administration of B. breve strains helps in restoring the healthy percentage of main microbial components.
Keywords: Bifidobacterium breve; coeliac disease; gluten free diet; intestinal microbiota; next generation sequencing; probiotic; qPCR.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Bifidobacterium breve Bb-03 | Balanced Firmicutes/Bacteroidetes Ratio | Beneficial | Moderate |
| Bifidobacterium breve Bb-03 | Improved Gut Microbiota Balance | Beneficial | Moderate |
| Bifidobacterium breve Bb-03 | Increased Levels of Actinobacteria | Beneficial | Moderate |
| Bifidobacterium breve BBr60 | Restored Healthy Gut Microbial Balance | Beneficial | Moderate |
| Bifidobacterium breve Bbr8 | Increased Levels of Actinobacteria | Beneficial | Moderate |
| Bifidobacterium breve Bbr8 | Reduced Firmicutes/Bacteroidetes Ratio | Beneficial | Moderate |
| Bifidobacterium breve BR-03 | Balanced Firmicutes/Bacteroidetes Ratio | Beneficial | Moderate |
| Bifidobacterium breve BR-03 | Improved Gut Microbiota Balance | Beneficial | Moderate |
| Bifidobacterium breve BR-03 | Increased Levels of Actinobacteria | Beneficial | Moderate |
| Bifidobacterium breve BR03 | Balanced Firmicutes/Bacteroidetes Ratio | Beneficial | Moderate |
| Bifidobacterium breve BR03 | Improved Gut Microbiota Balance | Beneficial | Moderate |
| Bifidobacterium breve BR03 | Increased Levels of Actinobacteria | Beneficial | Moderate |
| Bifidobacterium breve M-16V | Improved Gut Microbiota Balance | Beneficial | Moderate |
| Bifidobacterium breve M-16V | Increased Levels of Actinobacteria | Beneficial | Moderate |
| Bifidobacterium breve M-16V | Reduced Firmicutes/Bacteroidetes Ratio | Beneficial | Moderate |
| Bifidobacterium breve MAK40B22B | Improved Gut Microbiota Balance | Beneficial | Moderate |
| Bifidobacterium breve MAK40B22B | Increased Levels of Actinobacteria | Beneficial | Moderate |
| Bifidobacterium breve MAK40B22B | Reduced Firmicutes/Bacteroidetes Ratio | Beneficial | Moderate |
| Bifidobacterium breve R0070 | Improved Gut Microbiota Balance | Beneficial | Moderate |
| Bifidobacterium breve R0070 | Increased Levels of Actinobacteria | Beneficial | Moderate |
| Bifidobacterium breve R0070 | Restored Gut Microbiota Balance | Beneficial | Moderate |
| Bifidobacterium breve Rosell-70 | Balanced Firmicutes/Bacteroidetes Ratio | Beneficial | Moderate |
| Bifidobacterium breve Rosell-70 | Improved Gut Microbiota Balance | Beneficial | Moderate |
| Bifidobacterium breve Rosell-70 | Increased Levels of Actinobacteria | Beneficial | Moderate |
| Bifidobacterium breve SD5206 | Increased Levels of Actinobacteria | Beneficial | Moderate |
| Bifidobacterium breve SD5206 | Reduced Firmicutes/Bacteroidetes Ratio | Beneficial | Moderate |
| Bifidobacterium breve UABbr-11 | Restored Healthy Gut Microbial Balance | Beneficial | Moderate |
| Bifidobacterium breve VPro 52 | Improved Gut Microbiota Balance | Beneficial | Moderate |
| Bifidobacterium breve VPro 52 | Increased Levels of Actinobacteria | Beneficial | Moderate |
| Bifidobacterium breve VPro 52 | Reduced Firmicutes/Bacteroidetes Ratio | Beneficial | Moderate |