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Effect of Bifidobacterium breve on the Intestinal Microbiota of Coeliac Children on a Gluten Free Diet: A Pilot Study

  • 2016-10-22
  • Nutrients 8(10)
    • Andrea Quagliariello
    • I. Aloisio
    • Nicole Bozzi Cionci
    • D. Luiselli
    • G. D’Auria
    • L. Martinez-Priego
    • David Pérez-Villarroya
    • T. Langerholc
    • Maša Primec
    • D. Mičetić-Turk
    • D. Di Gioia

Abstract

Coeliac disease (CD) is associated with alterations of the intestinal microbiota. Although several Bifidobacterium strains showed anti-inflammatory activity and prevention of toxic gliadin peptides generation in vitro, few data are available on their efficacy when administered to CD subjects. This study evaluated the effect of administration for three months of a food supplement based on two Bifidobacterium breve strains (B632 and BR03) to restore the gut microbial balance in coeliac children on a gluten free diet (GFD). Microbial DNA was extracted from faeces of 40 coeliac children before and after probiotic or placebo administration and 16 healthy children (Control group). Sequencing of the amplified V3-V4 hypervariable region of 16S rRNA gene as well as qPCR of Bidobacterium spp., Lactobacillus spp., Bacteroides fragilis group Clostridium__sensu stricto and enterobacteria were performed. The comparison between CD subjects and Control group revealed an alteration in the intestinal microbial composition of coeliacs mainly characterized by a reduction of the Firmicutes/Bacteroidetes ratio, of Actinobacteria and Euryarchaeota. Regarding the effects of the probiotic, an increase of Actinobacteria was found as well as a re-establishment of the physiological Firmicutes/Bacteroidetes ratio. Therefore, a three-month administration of B. breve strains helps in restoring the healthy percentage of main microbial components.

Keywords: Bifidobacterium breve; coeliac disease; gluten free diet; intestinal microbiota; next generation sequencing; probiotic; qPCR.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Bifidobacterium breve Bb-03Balanced Firmicutes/Bacteroidetes RatioBeneficial
Moderate
Bifidobacterium breve Bb-03Improved Gut Microbiota BalanceBeneficial
Moderate
Bifidobacterium breve Bb-03Increased Levels of ActinobacteriaBeneficial
Moderate
Bifidobacterium breve BBr60Restored Healthy Gut Microbial BalanceBeneficial
Moderate
Bifidobacterium breve Bbr8Increased Levels of ActinobacteriaBeneficial
Moderate
Bifidobacterium breve Bbr8Reduced Firmicutes/Bacteroidetes RatioBeneficial
Moderate
Bifidobacterium breve BR-03Balanced Firmicutes/Bacteroidetes RatioBeneficial
Moderate
Bifidobacterium breve BR-03Improved Gut Microbiota BalanceBeneficial
Moderate
Bifidobacterium breve BR-03Increased Levels of ActinobacteriaBeneficial
Moderate
Bifidobacterium breve BR03Balanced Firmicutes/Bacteroidetes RatioBeneficial
Moderate
Bifidobacterium breve BR03Improved Gut Microbiota BalanceBeneficial
Moderate
Bifidobacterium breve BR03Increased Levels of ActinobacteriaBeneficial
Moderate
Bifidobacterium breve M-16VImproved Gut Microbiota BalanceBeneficial
Moderate
Bifidobacterium breve M-16VIncreased Levels of ActinobacteriaBeneficial
Moderate
Bifidobacterium breve M-16VReduced Firmicutes/Bacteroidetes RatioBeneficial
Moderate
Bifidobacterium breve MAK40B22BImproved Gut Microbiota BalanceBeneficial
Moderate
Bifidobacterium breve MAK40B22BIncreased Levels of ActinobacteriaBeneficial
Moderate
Bifidobacterium breve MAK40B22BReduced Firmicutes/Bacteroidetes RatioBeneficial
Moderate
Bifidobacterium breve R0070Improved Gut Microbiota BalanceBeneficial
Moderate
Bifidobacterium breve R0070Increased Levels of ActinobacteriaBeneficial
Moderate
Bifidobacterium breve R0070Restored Gut Microbiota BalanceBeneficial
Moderate
Bifidobacterium breve Rosell-70Balanced Firmicutes/Bacteroidetes RatioBeneficial
Moderate
Bifidobacterium breve Rosell-70Improved Gut Microbiota BalanceBeneficial
Moderate
Bifidobacterium breve Rosell-70Increased Levels of ActinobacteriaBeneficial
Moderate
Bifidobacterium breve SD5206Increased Levels of ActinobacteriaBeneficial
Moderate
Bifidobacterium breve SD5206Reduced Firmicutes/Bacteroidetes RatioBeneficial
Moderate
Bifidobacterium breve UABbr-11Restored Healthy Gut Microbial BalanceBeneficial
Moderate
Bifidobacterium breve VPro 52Improved Gut Microbiota BalanceBeneficial
Moderate
Bifidobacterium breve VPro 52Increased Levels of ActinobacteriaBeneficial
Moderate
Bifidobacterium breve VPro 52Reduced Firmicutes/Bacteroidetes RatioBeneficial
Moderate
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