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Evidence-Based Supplement Research
Evidence-Based Supplement Research

Study Design

Type
Randomized Controlled Trial (RCT)
Population
24 healthy volunteers (age 20-25 years; 13 males) sleep-deprived overnight
Methods
Double-blind, placebo-controlled, counterbalanced, two-way crossover study; 200-mg dose of L-theanine vs placebo (150 ml of distilled water); computerised traffic-scene-based visual recognition reaction task
Blinding
Double-blind
Duration
one week apart (single dose, tested pre-dose and 45 min post-dose)

Background

L-theanine is a non-protein-forming amino acid found in tea. Limited evidence suggests that it improves selective attention. Sleep deprivation impairs attention and psychomotor reactions, affecting automobile driving. We aimed to determine whether L-theanine improves neurobehavioral measures of visual attention in acutely sleep-deprived healthy adults in a traffic-scene-based attention task.

Methods

In a double-blind, placebo-controlled, counterbalanced, two-way crossover study, we compared the effects of a 200-mg dose of L-theanine with a placebo (150 ml of distilled water) on a computerised, traffic-scene-based visual recognition reaction task in 24 healthy volunteers (age 20-25 years; 13 males) sleep-deprived overnight. The participants made speeded button-presses to imminent accident scenes (i.e. hits), while ignoring safe scenes. They were tested pre-dose and 45 min post-dose, each treatment administered one week apart.

Results

Hit rates were more than 90% in all sessions, and were similar in two treatments, pre- vs post-dose. L-theanine significantly reduced false alarms (i.e. responses to safe scenes) (p = 0.014) and increased A' (i.e. target-distractor discriminability) (p = 0.009), whereas placebo did not (p > 0.05). L-theanine reduced hit reaction time by 38.65 ms (p = 0.007), and placebo by 19.08 ms (p = 0.016), however reaction time changes from baseline were not significantly different between treatments (p > 0.05).

Conclusions

L-theanine in high doses appears to improve selective visual attention by concurrently improving information processing speed and target-distractor discriminability in acutely sleep-deprived individuals. This is consistent with previous functional neuroimaging findings, where L-theanine suppressed distractor-processing and default-mode-network activity in visual selective attention tasks.

Research Insights

  • Hit rates were more than 90% in all sessions, and were similar in two treatments, pre- vs post-dose.

    Effect
    Neutral
    Effect size
    Small
    Dose
    200 mg
  • increased A' (i.e. target-distractor discriminability) (p = 0.009), whereas placebo did not (p > 0.05)

    Effect
    Beneficial
    Effect size
    Small
    Dose
    200 mg
  • L-theanine significantly reduced false alarms (i.e. responses to safe scenes) (p = 0.014)

    Effect
    Beneficial
    Effect size
    Small
    Dose
    200 mg
  • L-theanine reduced hit reaction time by 38.65 ms (p = 0.007), and placebo by 19.08 ms (p = 0.016), however reaction time changes from baseline were not significantly different between treatments (p > 0.05)

    Effect
    Neutral
    Effect size
    Small
    Dose
    200 mg
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