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Abstract

Aim: To evaluate the ability of Lactobacillus rhamnosus HN001 and Bifidobacterium longum BB536 to colonize the intestinal environment of healthy subjects and modify the gut microbiota composition.

Methods: Twenty healthy Italian volunteers, eight males and twelve females, participated in the study. Ten subjects took a sachet containing 4 × 109 colony-forming units (CFU) of Bifidobacterium longum BB536 and 109 CFU of Lactobacillus rhamnosus HN001, 30 min before breakfast (pre-prandial administration), while ten subjects took a sachet of probiotic product 30 min after breakfast (post-prandial administration). The ability of Lactobacillus rhamnosus HN001 and Bifidobacterium longum BB536 to colonize human gut microbiota was assessed by means of quantitative real-time PCR, while changes in gut microbiota composition were detected by using Ion Torrent Personal Genome Machine.

Results: Immediately after 1-mo of probiotic administration, B. longum BB536 and L. rhamnosus HN001 load was increased in the majority of subjects in both pre-prandial and post-prandial groups. This increase was found also 1 mo after the end of probiotic oral intake in both groups, if compared to samples collected before probiotic consumption. At phyla level a significant decrease in Firmicutes abundance was detected immediately after 1-mo of B. longum BB536 and L. rhamnosus HN001 oral intake. This reduction persisted up to 1 mo after the end of probiotic oral intake together with a significant decrease of Proteobacteria abundance if compared to samples collected before probiotic administration. Whereas, at species level, a higher abundance of Blautia producta, Blautia wexlerae and Haemophilus ducrey was observed, together with a reduction of Holdemania filiformis, Escherichia vulneris, Gemmiger formicilis and Streptococcus sinensis abundance. In addition, during follow-up period we observed a further reduction in Escherichia vulneris and Gemmiger formicilis, together with a decrease in Roseburia faecis and Ruminococcus gnavus abundance. Conversely, the abundance of Akkermansia muciniphila was increased if compared to samples collected at the beginning of the experimental time course.

Conclusion: B. longum BB536 and L. rhamnosus HN001 showed the ability to modulate the gut microbiota composition, leading to a significant reduction of potentially harmful bacteria and an increase of beneficial ones. Further studies are needed to better understand the specific mechanisms involved in gut microbiota modulation.

Keywords: Bifidobacterium; Gut microbiota; Human health; Lactobacillus; Probiotics.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Bifidobacterium longum BB536Improved Gut Microbiota CompositionBeneficial
Moderate
Bifidobacterium longum BB536Increased Abundance of Beneficial Bacterial GeneraBeneficial
Moderate
Bifidobacterium longum BB536Increased Beneficial Gut BacteriaBeneficial
Moderate
Bifidobacterium longum BB536Reduced Abundance of Harmful BacteriaBeneficial
Large
Bifidobacterium longum BB536Reduced Harmful BacteriaBeneficial
Moderate
Bifidobacterium longum SP54Increased Akkermansia muciniphila AbundanceBeneficial
Moderate
Bifidobacterium longum SP54Increased Blautia Producta AbundanceBeneficial
Moderate
Bifidobacterium longum SP54Increased Blautia Wexlerae AbundanceBeneficial
Moderate
Bifidobacterium longum SP54Reduced Abundance of ProteobacteriaBeneficial
Moderate
Bifidobacterium longum SP54Reduced Escherichia Vulneris AbundanceBeneficial
Moderate
Bifidobacterium longum SP54Reduced Firmicutes AbundanceBeneficial
Moderate
Bifidobacterium longum SP54Reduced Gemmiger formicilis AbundanceBeneficial
Moderate
Lactobacillus rhamnosusIncreased Akkermansia muciniphila AbundanceBeneficial
Moderate
Lactobacillus rhamnosusIncreased Beneficial Gut BacteriaBeneficial
Moderate
Lactobacillus rhamnosusReduced Abundance of ProteobacteriaBeneficial
Moderate
Lactobacillus rhamnosusReduced Escherichia Vulneris AbundanceBeneficial
Moderate
Lactobacillus rhamnosusReduced Firmicutes AbundanceBeneficial
Moderate
Lactobacillus rhamnosusReduced Harmful BacteriaBeneficial
Moderate
Lactobacillus rhamnosus HA-500Enhanced Gut Microbiota CompositionBeneficial
Moderate
Lactobacillus rhamnosus HA-500Reduced Abundance of ProteobacteriaBeneficial
Moderate
Lactobacillus rhamnosus HA-500Reduced Firmicutes AbundanceBeneficial
Moderate
Lactobacillus rhamnosus HA-500Reduced Harmful Bacteria SpeciesBeneficial
Large
Lactobacillus rhamnosus NH001Increased Beneficial Gut MicrobiotaBeneficial
Moderate
Lactobacillus rhamnosus NH001Reduced Harmful Gut BacteriaBeneficial
Moderate
Lactobacillus rhamnosus R0011Increased Beneficial Gut BacteriaBeneficial
Moderate
Lactobacillus rhamnosus R0011Modulated Gut Microbiota CompositionBeneficial
Moderate
Lactobacillus rhamnosus R0011Reduced Harmful BacteriaBeneficial
Moderate

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