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Effect of Lactobacillus rhamnosus on the response of Galleria mellonella against Staphylococcus aureus and Escherichia coli infections

  • 2017-11-24
  • Archives of Microbiology 200(3)
    • A. L. Jorjão
    • F. E. Oliveira
    • M. V. P. Leão
    • A. O. Jorge
    • L. D. Oliveira

Abstract

This study evaluated the prophylactic effects of the live or heat-killed probiotic strain Lactobacillus rhamnosus ATCC 7469 in Galleria mellonella, inoculated with Staphylococcus aureus or Escherichia coli. L. rhamnosus suspension was prepared and a part of it was autoclaved to obtain heat-killed lactobacilli. The larvae were inoculated of these suspensions and pathogenic. The survival of the larvae was observed during 7 days and after 24 h of inoculation haemocytes counted, melanization and nitric oxide production were analyzed. Larvae survival rate increased in the group inoculated with heat-killed L. rhamnosus, however, with no statistical difference. There was a significant increase in total haemocyte counts in all test groups. Haemolymph melanization and nitric oxide production were higher in the group inoculated with L. rhamnosus and infected with S. aureus. It was concluded that, in this model of infection, heat-killed L. rhamnosus ATCC 7469 promoted greater protection in Galleria mellonella infected with S. aureus or E. coli.

Keywords: Curve survival; Galleria mellonella; Infection; Lactobacillus rhamnosus; Melanization.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus rhamnosus HA-11Improved Larvae Survival RateNeutral
Small
Lactobacillus rhamnosus HA-11Increased Total Hemocyte CountsBeneficial
Large
Lactobacillus rhamnosus HA-114Improved Larvae Survival RateNeutral
Small
Lactobacillus rhamnosus HA-114Increased Haemolymph MelanizationBeneficial
Moderate
Lactobacillus rhamnosus HA-114Increased Nitric Oxide ProductionBeneficial
Moderate
Lactobacillus rhamnosus HA-114Increased Total Hemocyte CountsBeneficial
Large
Lactobacillus rhamnosus HA-500Enhanced MelanizationBeneficial
Moderate
Lactobacillus rhamnosus HA-500Improved Cell Survival RatesBeneficial
Small
Lactobacillus rhamnosus HA-500Increased Nitric Oxide ProductionBeneficial
Moderate
Lactobacillus rhamnosus HA-500Increased Total Hemocyte CountsBeneficial
Large
Lactobacillus rhamnosus LCS-742Improved Larvae Survival RateNeutral
Small
Lactobacillus rhamnosus LCS-742Increased Haemolymph MelanizationBeneficial
Moderate
Lactobacillus rhamnosus LCS-742Increased Nitric Oxide ProductionBeneficial
Moderate
Lactobacillus rhamnosus LCS-742Increased Total Hemocyte CountsBeneficial
Large
Lactobacillus rhamnosus LR06Improved Infection ProtectionBeneficial
Moderate
Lactobacillus rhamnosus LR06Improved Larvae Survival RateNeutral
Small
Lactobacillus rhamnosus LR06Increased Haemolymph MelanizationBeneficial
Moderate
Lactobacillus rhamnosus LR06Increased Hemocyte CountBeneficial
Moderate
Lactobacillus rhamnosus LR06Increased Nitric Oxide ProductionBeneficial
Moderate

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