Effect of Lactobacillus rhamnosus on the response of Galleria mellonella against Staphylococcus aureus and Escherichia coli infections
- 2017-11-24
- Archives of Microbiology 200(3)
- A. L. Jorjão
- F. E. Oliveira
- M. V. P. Leão
- A. O. Jorge
- L. D. Oliveira
- PubMed: 29177947
- DOI: 10.1007/s00203-017-1441-7
Abstract
This study evaluated the prophylactic effects of the live or heat-killed probiotic strain Lactobacillus rhamnosus ATCC 7469 in Galleria mellonella, inoculated with Staphylococcus aureus or Escherichia coli. L. rhamnosus suspension was prepared and a part of it was autoclaved to obtain heat-killed lactobacilli. The larvae were inoculated of these suspensions and pathogenic. The survival of the larvae was observed during 7 days and after 24 h of inoculation haemocytes counted, melanization and nitric oxide production were analyzed. Larvae survival rate increased in the group inoculated with heat-killed L. rhamnosus, however, with no statistical difference. There was a significant increase in total haemocyte counts in all test groups. Haemolymph melanization and nitric oxide production were higher in the group inoculated with L. rhamnosus and infected with S. aureus. It was concluded that, in this model of infection, heat-killed L. rhamnosus ATCC 7469 promoted greater protection in Galleria mellonella infected with S. aureus or E. coli.
Keywords: Curve survival; Galleria mellonella; Infection; Lactobacillus rhamnosus; Melanization.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus rhamnosus HA-11 | Improved Larvae Survival Rate | Neutral | Small |
| Lactobacillus rhamnosus HA-11 | Increased Total Hemocyte Counts | Beneficial | Large |
| Lactobacillus rhamnosus HA-114 | Improved Larvae Survival Rate | Neutral | Small |
| Lactobacillus rhamnosus HA-114 | Increased Haemolymph Melanization | Beneficial | Moderate |
| Lactobacillus rhamnosus HA-114 | Increased Nitric Oxide Production | Beneficial | Moderate |
| Lactobacillus rhamnosus HA-114 | Increased Total Hemocyte Counts | Beneficial | Large |
| Lactobacillus rhamnosus HA-500 | Enhanced Melanization | Beneficial | Moderate |
| Lactobacillus rhamnosus HA-500 | Improved Cell Survival Rates | Beneficial | Small |
| Lactobacillus rhamnosus HA-500 | Increased Nitric Oxide Production | Beneficial | Moderate |
| Lactobacillus rhamnosus HA-500 | Increased Total Hemocyte Counts | Beneficial | Large |
| Lactobacillus rhamnosus LCS-742 | Improved Larvae Survival Rate | Neutral | Small |
| Lactobacillus rhamnosus LCS-742 | Increased Haemolymph Melanization | Beneficial | Moderate |
| Lactobacillus rhamnosus LCS-742 | Increased Nitric Oxide Production | Beneficial | Moderate |
| Lactobacillus rhamnosus LCS-742 | Increased Total Hemocyte Counts | Beneficial | Large |
| Lactobacillus rhamnosus LR06 | Improved Infection Protection | Beneficial | Moderate |
| Lactobacillus rhamnosus LR06 | Improved Larvae Survival Rate | Neutral | Small |
| Lactobacillus rhamnosus LR06 | Increased Haemolymph Melanization | Beneficial | Moderate |
| Lactobacillus rhamnosus LR06 | Increased Hemocyte Count | Beneficial | Moderate |
| Lactobacillus rhamnosus LR06 | Increased Nitric Oxide Production | Beneficial | Moderate |