Effect of Long-Term Consumption of Lactobacillus paracasei SD1 on Reducing Mutans streptococci and Caries Risk: A Randomized Placebo-Controlled Trial
- 2015-04-01
- Dentistry Journal 3(2)
- R. Teanpaisan
- S. Piwat
- S. Tianviwat
- B. Sophatha
- Thanyanan Kampoo
- PubMed: 29567924
- DOI: 10.3390/dj3020043
Abstract
Background: A previous study revealed Lactobacillus paracasei SD1, a probiotic strain, could reduce mutans streptococci (MS). The aim of this study was to evaluate the long-term effects of L. paracasei SD1 on the colonization of MS, and whether caries lesions developed.
Methods: After informed consent, 122 children were recruited and randomly assigned to the probiotic or control groups. The probiotic group received milk-powder containing L. paracasei SD1 and the control group received standard milk-powder once daily for six months. Salivary MS and lactobacilli were enumerated using differential culture at baseline and at three-month intervals for 12 months. The persistence of L. paracasei SD1 was investigated using AP-PCR for DNA-fingerprinting. Oral health was examined at baseline and at the end of the study according to WHO criteria.
Results: The long-term consumption could prolong colonization of L. paracasei SD1. Significantly reduced MS counts and increased lactobacilli levels were found among children in the probiotic group. There were less caries lesions in the probiotic group at the end of the study. A significant reduction of the development of new caries lesions (4.5 times) was observed in the high caries risk group but not in the low caries risk group.
Conclusions: Results demonstrate that the long-term daily ingestion of the human-derived probiotic L. paracasei SD1 significantly reduces the number of MS and caries risk in the high caries group.
Keywords: Lactobacillus paracasei SD1; dental caries; mutans streptococci; probiotics.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus casei SD-5213 | Reduced Dental Caries | Beneficial | Large |
| Lactobacillus casei SD-5213 | Reduced Mutans Streptococcus Counts | Beneficial | Large |
| Lactobacillus paracasei 431 | Reduced Caries Lesions | Beneficial | Moderate |
| Lactobacillus paracasei 431 | Reduced Mutans Streptococcus Counts | Beneficial | Large |
| Lactobacillus paracasei DSM 13434 | Reduced Caries Lesions | Beneficial | Moderate |
| Lactobacillus paracasei DSM 13434 | Reduced Caries Risk | Beneficial | Large |
| Lactobacillus paracasei DSM 13434 | Reduced Mutans Streptococci Levels | Beneficial | Large |
| Lactobacillus paracasei HA-196 | Reduced Caries Incidence | Beneficial | Large |
| Lactobacillus paracasei HA-196 | Reduced Salivary Levels of Mutans Streptococci | Beneficial | Large |
| Lactobacillus paracasei IMC 502 | Reduced Dental Caries | Beneficial | Large |
| Lactobacillus paracasei IMC 502 | Reduced Mutans Streptococci Levels | Beneficial | Large |
| Lactobacillus paracasei subsp. paracasei UALpc-04 | Reduced Dental Caries | Beneficial | Large |
| Lactobacillus paracasei subsp. paracasei UALpc-04 | Reduced Mutans Streptococci Colonization | Beneficial | Moderate |
| Lactobacillus paracasei subsp. paracasei UALs-04 | Increased Colonization by Lactobacillus paracasei SD1 | Beneficial | Moderate |
| Lactobacillus paracasei subsp. paracasei UALs-04 | Reduced Caries Risk | Beneficial | Large |
| Lactobacillus paracasei UALpc-04 | Reduced Mutans Streptococci Levels | Beneficial | Large |
| Lactobacillus paracasei VPro 224 | Increased Lactobacilli Levels | Beneficial | Large |
| Lactobacillus paracasei VPro 224 | Reduced Caries Lesions | Beneficial | Moderate |
| Lactobacillus paracasei VPro 224 | Reduced Salivary Levels of Mutans Streptococci | Beneficial | Large |