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Effect of Multi-strain Probiotic Formulation on Students Facing Examination Stress: a Double-Blind, Placebo-Controlled Study

  • 2020-06-29
  • Probiotics and Antimicrobial Proteins 13(1)
    • R. Venkataraman
    • R. Madempudi
    • J. Neelamraju
    • J. Ahire
    • H. Vinay
    • Anila Lal
    • Glory Thomas
    • S. Stephen


In this placebo-controlled, double-blind clinical trial, we have investigated the effect of multi-strain probiotic (Bacillus coagulans Unique IS2, Lactobacillus rhamnosus UBLR58, Bifidobacterium lactis UBBLa70, Lactobacillus plantarum UBLP40 (each of 2 billion CFU); Bifidobacterium breve UBBr01, Bifidobacterium infantis UBBI01 (each of 1 billion CFU)) capsule with glutamine (250 mg) on students facing examination stress. A total of 80 students (18-24 years) were enrolled and randomised to receive multi-strain probiotic or placebo capsules twice a day for 28 days (i.e. pre- and during examination). The stress was analysed at the baseline and the end of the treatment by using the perceived stress scale (PSS), depression anxiety stress scale (DASS), and state-trait anxiety inventory (STAI) questionnaire. The serum cortisol levels were also determined. As a result, at the end of the trial, a total of 74 students completed the study, and those who consumed probiotic capsules showed a significant reduction in PSS, DASS, and STAI scores, and serum cortisol levels from the baseline as compared with placebo. No adverse events were reported during the study. In conclusion, the multi-strain probiotic is effective in reducing stress associated with examination. CTRI/2019/03/018178.

Keywords: Anxiety; Clinical trial; Cortisol; Multi-strain; Probiotic; Stress.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Bacillus coagulans LactoSpore 1 billion CFUReduced Subjective Stress LevelsBeneficial
Bacillus coagulans Unique IS-2Reduced Stress LevelsBeneficial

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