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Introduction

A systematic literature search was undertaken to assess the impact of pre-, pro-, and syn-biotic supplementation on measures of gastrointestinal status at rest and in response to acute exercise.

Methods

Six databases (Ovid MEDLINE, EMBASE, Cinahl, SportsDISCUS, Web of Science, and Scopus) were used. Included were human research studies in healthy sedentary adults, and healthy active adults, involving supplementation and control or placebo groups. Sedentary individuals with non-communicable disease risk or established gastrointestinal inflammatory or functional diseases/disorders were excluded.

Results

A total of n = 1,204 participants were included from n = 37 papers reported resting outcomes, and n = 13 reported exercise-induced gastrointestinal syndrome (EIGS) outcomes. No supplement improved gastrointestinal permeability or gastrointestinal symptoms (GIS), and systemic endotoxemia at rest. Only modest positive changes in inflammatory cytokine profiles were observed in n = 3/15 studies at rest. Prebiotic studies (n = 4/5) reported significantly increased resting fecal Bifidobacteria, but no consistent differences in other microbes. Probiotic studies (n = 4/9) increased the supplemented bacterial species-strain. Only arabinoxylan oligosaccharide supplementation increased total fecal short chain fatty acid (SCFA) and butyrate concentrations. In response to exercise, probiotics did not substantially influence epithelial injury and permeability, systemic endotoxin profile, or GIS. Two studies reported reduced systemic inflammatory cytokine responses to exercise. Probiotic supplementation did not substantially influence GIS during exercise.

Discussion

Synbiotic outcomes resembled probiotics, likely due to the minimal dose of prebiotic included. Methodological issues and high risk of bias were identified in several studies, using the Cochrane Risk of Bias Assessment Tool. A major limitation in the majority of included studies was the lack of a comprehensive approach of well-validated biomarkers specific to gastrointestinal outcomes and many included studies featured small sample sizes. Prebiotic supplementation can influence gut microbial composition and SCFA concentration; whereas probiotics increase the supplemented species-strain, with minimal effect on SCFA, and no effect on any other gastrointestinal status marker at rest. Probiotic and synbiotic supplementation does not substantially reduce epithelial injury and permeability, systemic endotoxin and inflammatory cytokine profiles, or GIS in response to acute exercise.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus rhamnosus lysate powderAltered Gut Microbial Community CompositionBeneficial
Small
Lactobacillus rhamnosus lysate powderAltered Pro-Inflammatory Cytokine LevelsBeneficial
Small
Lactobacillus rhamnosus lysate powderExercise-Induced Gastrointestinal SymptomsNeutral
Small
Lactobacillus rhamnosus lysate powderReduced Gastrointestinal SymptomsNeutral
Small
Lactobacillus rhamnosus lysate powderReduced Intestinal PermeabilityNeutral
Small
Lactobacillus rhamnosus lysate powderReduced Metabolic EndotoxemiaNeutral
Small
Lactobacillus rhamnosus lysate powderReduced Systemic Endotoxemia at RestNeutral
Small
Lactobacillus salivarius UCC118Increased Abundance of Specific Bacterial SpeciesBeneficial
Small
Lactobacillus salivarius UCC118Increased Fecal Probiotic StrainsBeneficial
Small
Lactobacillus salivarius UCC118Increased Gut Microbial SupplementationBeneficial
Small
Lactobacillus salivarius UCC118No Improvement in Gastrointestinal PermeabilityNeutral
Small
Lactobacillus salivarius UCC118No Substantial Reduction in Exercise-Induced Gastrointestinal Injury, Permeability, Endotoxin Response, or Gastrointestinal SymptomsNeutral
Small
Lactobacillus salivarius UCC118Unchanged Gastrointestinal SymptomsNeutral
Small
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