Effects and Mechanisms of Resveratrol on the Adhesion of Lactobacillus acidophilus NCFM.

2022-11-14
Probiotics and antimicrobial proteins 15(6)

PubMed: 36376613
DOI: 10.1007/s12602-022-10007-9

Study Design

Population: Lactobacillus acidophilus NCFM and HT-29 cells
Methods: Five common polyphenols in fruits and vegetables, including resveratrol, epicatechin, quercetin, hesperidin, and caffeic acid, were screened; adhesion to mucin, surface hydrophobicity, auto-aggregation, and proteomic analysis were assessed

Rigorous Journal

Based on the adhesion and surface properties of Lactobacillus acidophilus NCFM, five common polyphenols in fruits and vegetables, including resveratrol, epicatechin, quercetin, hesperidin, and caffeic acid, were screened, and the reasons for resveratrol promoting adhesion were systematically explained. The results showed that resveratrol could significantly enhance NCFM adhesion to mucin (1.73 fold), followed by epicatechin (1.47 fold), caffeic acid (1.30 fold), and hesperidin (0.99 fold), while quercetin had a certain degree of inhibition (0.84 fold). The effects of these polyphenols on surface hydrophobicity and auto-aggregation of NCFM were consistent with adhesion results. Then, how resveratrol promotes NCFM adhesion was further explored. The results of the proteomic analysis showed that resveratrol changed the surface layer proteins of NCFM, involving 4 up-regulated proteins and 12 down-regulated proteins. In addition, resveratrol promoted the expression of mucin genes and the glycosylation of mucins on the HT-29 cell surface. Our results indicate that resveratrol changes the surface layer proteins of NCFM to modify surface properties and adhere to mucins. Meanwhile, resveratrol promotes expression and glycosylation of mucins in HT-29 cells. Our findings provide theoretical support for an in-depth explanation of the interaction among resveratrol, NCFM, and the HT-29 cells.

Research Insights

Supplement	Dose	Health Outcome	Effect Type	Effect Size	Source
Lactobacillus acidophilus NCFM	—	Increased Cell Adhesion	Beneficial	Moderate	View source resveratrol could significantly enhance NCFM adhesion to mucin (1.73 fold) ... The effects of these polyphenols on surface hydrophobicity and auto-aggregation of NCFM were consistent with adhesion results.