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Abstract

In the present study, we aimed to investigate the modulatory effects of a potential probiotic bacterium Lactobacillus gasseri ATCC 33323 on Helicobacter pylori-induced inflammatory response and gene expression in human gastric adenocarcinoma (AGS) cell line. The gastric epithelial cells were coinfected with a collection of H. pylori clinical strains alone or in combination with L. gasseri at a multiplicity of infection (MOI) of 1:100 for each bacterium, and incubated for different time points of 3, 6, and 12 h. IL-8 secretion from coinfected AGS cells after incubation at each time point was measured by an enzyme-linked immunosorbent assay (ELISA). The mRNA expression of IL-8, Bcl-2, β-catenin, integrin α5, and integrin β1 genes was determined by quantitative RT-PCR amplification of total RNA extracted from coinfected epithelial cells. L. gasseri significantly (P < 0.05 and P < 0.01) decreased the production of IL-8 in AGS cells coinfected with H. pylori strains at 6 h post-infection. We also detected that L. gasseri significantly (P < 0.05) down-regulated the gene expression level of IL-8 in H. pylori-stimulated AGS cells after 6 and 12 h of coinfection. Similarly, L. gasseri caused a significant decrease (P < 0.05) in mRNA expression of Bcl-2, β-catenin, integrin α5, and integrin β1 genes in AGS cells at 3 and 6 h after infection with H. pylori strains as compared with non-infected control cells. In conclusion, our results demonstrated that L. gasseri ameliorates H. pylori-induced inflammation and could be developed as a supplementation to the current treatment regimens administrated against H. pylori infection.

Keywords: Coinfection; Helicobacter pylori; IL-8; Inflammation; Lactobacillus gasseri ATCC 33323; Probiotic.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus gasseriReduced Inflammation LevelsBeneficial
Moderate
Lactobacillus gasseriReduced Pro-inflammatory Gene ExpressionBeneficial
Moderate
Lactobacillus gasseri BNR17Reduced InflammationBeneficial
Moderate
Lactobacillus gasseri KS-13Reduced Expression of Inflammation-Related GenesBeneficial
Moderate
Lactobacillus gasseri KS-13Reduced Gene ExpressionBeneficial
Moderate
Lactobacillus gasseri KS-13Reduced Inflammatory ResponseBeneficial
Moderate
Lactobacillus gasseri LAC-343Reduced IL-8 Gene ExpressionBeneficial
Moderate
Lactobacillus gasseri LAC-343Reduced IL-8 ProductionBeneficial
Moderate
Lactobacillus gasseri LBV 150NReduced Inflammatory Gene Expression in Helicobacter pylori-Stimulated CellsBeneficial
Moderate
Lactobacillus gasseri LBV 150NReduced Inflammatory Response to Helicobacter pyloriBeneficial
Moderate
Lactobacillus gasseri LQ-36Reduced IL-8 Production in Gastric Epithelial CellsBeneficial
Moderate
Lactobacillus gasseri LQ-36Reduced Pro-inflammatory Gene ExpressionBeneficial
Moderate
Lactobacillus gasseri SD-5585Decreased Integrin β1 mRNA ExpressionBeneficial
Moderate
Lactobacillus gasseri SD-5585Reduced IL-8 Gene ExpressionBeneficial
Moderate
Lactobacillus gasseri SD-5585Reduced IL-8 ProductionBeneficial
Moderate
Lactobacillus gasseri SD-5585Reduced mRNA Expression of Bcl-2Beneficial
Moderate
Lactobacillus gasseri SD-5585Reduced mRNA Expression of Integrin α5Beneficial
Moderate
Lactobacillus gasseri SD-5585Reduced mRNA Expression of β-cateninBeneficial
Moderate
Lactobacillus gasseri UALg-05Reduced Expression of Inflammation-Related GenesBeneficial
Moderate
Lactobacillus gasseri UALg-05Reduced Inflammation LevelsBeneficial
Moderate

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