Skip to main content

Effects of Bifidobacterium longum BB536 and Lactobacillus rhamnosus HN001 in IBS patients

  • 2020-02-12
  • European Journal of Clinical Investigation 50(3)
    • L. Bonfrate
    • D. D. Di Palo
    • G. Celano
    • A. Albert
    • Paola Vitellio
    • M. De Angelis
    • M. Gobbetti
    • P. Portincasa


Background: Irritable bowel syndrome (IBS) is a common gastrointestinal disorder, which still lacks effective therapy. We aimed to investigate the effects of a novel formulation of Bifidobacterium longum BB536 and Lactobacillus rhamnosus HN001 with vitamin B6 (LBB) on symptoms, intestinal permeability, cultivable bacteria and metabolome in IBS subjects.

Materials and methods: Twenty-five IBS patients (Rome IV criteria) (M:F = 8:17; age 48 years ± 11 SD) were randomized to treatment (LBB) or placebo (one month each) in a crossover randomized double-blind controlled trial. Symptoms, intestinal habits, disease severity, intestinal permeability and intestinal microbiota were analysed at 0, 30, 45 and 60 days.

Results: Percentage decrease from baseline of abdominal pain (-48.8% vs -3.5%), bloating (-36.35% vs +7.35%) and severity of disease (-30.1% vs -0.4%) was significantly (P < .0001) greater with LBB than placebo, respectively. In IBS-D patients, the improvement from baseline of Bristol score was more consistent with LBB (from 6 ± 0.4 to 4.3 ± 1.1, P < .00001) than placebo (from 6.2 ± 0.7 to 5.3 ± 1.1, P = .04). In IBS-C patients, Bristol score tended to improve from baseline after LBB (2.6 ± 1.1 vs 3.2 ± 0.5, P = .06). LBB significantly improved the percentage of sucralose recovery (colonic permeability) (1.86 ± 0.1 vs 1.1 ± 0.2, P = .01). During treatment, presumptive lactic acid bacteria and bifidobacteria, relative abundance of propanoic, butanoic, pentanoic acids and hydrocarbons increased, while phenol decreased.

Conclusions: The novel formulation of B. longum BB536 and L. rhamnosus HN001 with B6 vitamin improves symptoms and severity of disease, restores intestinal permeability and gut microbiota in IBS patients.

Keywords: abdominal pain; bloating; functional gastrointestinal disorders; randomized placebo-controlled study.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Bifidobacterium longum BB536Enhanced Beneficial Metabolite ProductionBeneficial
Bifidobacterium longum BB536Improved Stool ConsistencyBeneficial
Bifidobacterium longum BB536Reduced Abdominal PainBeneficial
Bifidobacterium longum BB536Reduced BloatingBeneficial
Bifidobacterium longum BB536Reduced Colonic PermeabilityBeneficial

Pillser helps you make informed health decisions by providing comprehensive, unbiased information about supplements. This includes detailed research on supplement ingredients, their benefits, potential risks, and their overall efficacy. You can contribute by sharing your feedback and suggestions.

Send us an email at