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Effects of dietary supplementation of Lactobacillus rhamnosus or/and Lactococcus lactis on the growth, gut microbiota and immune responses of red sea bream, Pagrus major.

  • 2016-02
  • Fish & Shellfish Immunology 49
    • M. Dawood
    • S. Koshio
    • M. Ishikawa
    • S. Yokoyama
    • M. F. El Basuini
    • Md. Sakhawat Hossain
    • Truong H. Nhu
    • Serge Dossou
    • Amina S. Moss

Abstract

Pagrus major fingerlings (3·29 ± 0·02 g) were fed with basal diet (control) supplemented with Lactobacillus rhamnosus (LR), Lactococcus lactis (LL), and L. rhamnosus + L. lactis (LR + LL) at 10(6) cell g(-1) feed for 56 days. Feeding a mixture of LR and LL significantly increased feed utilization (FER and PER), intestine lactic acid bacteria (LAB) count, plasma total protein, alternative complement pathway (ACP), peroxidase, and mucus secretion compared with the other groups (P < 0.05). Serum lysozyme activity (LZY) significantly increased in LR + LL when compared with the control group. Additionally, fish fed the LR + LL diet showed a higher growth performance (Fn wt, WG, and SGR) and protein digestibility than the groups fed an individual LR or the control diet. Superoxide dismutase (SOD) significantly increased in LR and LR + LL groups when compared with the other groups. Moreover, the fish fed LR or LL had better improvement (P < 0.05) in growth, feed utilization, body protein and lipid contents, digestibility coefficients (dry matter, protein, and lipid), protease activity, total intestine and LAB counts, hematocrit, total plasma protein, biological antioxidant potential, ACP, serum and mucus LZY and bactericidal activities, peroxidase, SOD, and mucus secretion than the control group. Interestingly, fish fed diets with LR + LL showed significantly lower total cholesterol and triglycerides when compared with the other groups (P < 0.05). These data strongly suggest that a mixture of LR and LL probiotics may serve as a healthy immunostimulating feed additive in red sea bream aquaculture.

Keywords: Feed utilization; Growth; Immunity; Lactobacillus rhamnosus; Lactococcus lactis; Red sea bream.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus lactis LLa61Enhanced Antioxidant CapacityBeneficial
Large
Lactobacillus lactis LLa61Enhanced Growth PerformanceBeneficial
Large
Lactobacillus lactis LLa61Improved Alternative Complement Pathway ActivityBeneficial
Large
Lactobacillus lactis LLa61Improved Apparent Digestibility of Crude ProteinBeneficial
Large
Lactobacillus lactis LLa61Improved Digestibility CoefficientsBeneficial
Large
Lactobacillus lactis LLa61Improved Feed UtilizationBeneficial
Large
Lactobacillus lactis LLa61Increased Hematocrit LevelsBeneficial
Large
Lactobacillus lactis LLa61Increased Lactic Acid Bacteria LevelsBeneficial
Large
Lactobacillus lactis LLa61Increased Mucus SecretionBeneficial
Large
Lactobacillus lactis LLa61Increased Peroxidase LevelsBeneficial
Large
Lactobacillus lactis LLa61Increased Plasma Total ProteinBeneficial
Large
Lactobacillus lactis LLa61Increased Protease ActivityBeneficial
Large
Lactobacillus lactis LLa61Increased Serum Lysozyme ActivityBeneficial
Moderate
Lactobacillus lactis LLa61Increased Superoxide Dismutase LevelsBeneficial
Large
Lactobacillus rhamnosus LR04Improved Alternative Complement Pathway ActivityBeneficial
Large
Lactobacillus rhamnosus LR04Improved Feed UtilizationBeneficial
Large
Lactobacillus rhamnosus LR04Improved Growth PerformanceBeneficial
Large
Lactobacillus rhamnosus LR04Improved Plasma Total ProteinBeneficial
Large
Lactobacillus rhamnosus LR04Increased Intestinal Lactic Acid Bacteria CountBeneficial
Large
Lactobacillus rhamnosus LR04Increased Mucus SecretionBeneficial
Large
Lactobacillus rhamnosus LR04Increased Peroxidase ActivityBeneficial
Large
Lactobacillus rhamnosus LR04Increased Serum Lysozyme ActivityBeneficial
Moderate
Lactobacillus rhamnosus LR04Increased Superoxide Dismutase ActivityBeneficial
Moderate
Lactobacillus rhamnosus LR06Enhanced Immune ResponseBeneficial
Large
Lactobacillus rhamnosus LR06Improved Feed UtilizationBeneficial
Large
Lactobacillus rhamnosus LR06Improved Growth PerformanceBeneficial
Large
Lactobacillus rhamnosus LR06Increased Gut Lactic Acid Bacteria CountBeneficial
Large
Lactobacillus rhamnosus LR06Increased Protein DigestibilityBeneficial
Large
Lactobacillus rhamnosus LR06Increased Serum Lysozyme ActivityBeneficial
Moderate
Lactobacillus rhamnosus LR06Increased Superoxide Dismutase ActivityBeneficial
Moderate
Lactobacillus rhamnosus Lr-32Improved Feed UtilizationBeneficial
Large
Lactobacillus rhamnosus Lr-32Improved Fish Chemical CompositionBeneficial
Moderate
Lactobacillus rhamnosus Lr-32Improved Plasma Total ProteinBeneficial
Large
Lactobacillus rhamnosus Lr-32Increased Complement Pathway ActivityBeneficial
Large
Lactobacillus rhamnosus Lr-32Increased Intestinal Lactic Acid Bacteria CountBeneficial
Large
Lactobacillus rhamnosus Lr-32Increased Mucus SecretionBeneficial
Large
Lactobacillus rhamnosus Lr-32Increased Peroxidase ActivityBeneficial
Large
Lactobacillus rhamnosus Lr-32Increased Serum Lysozyme ActivityBeneficial
Moderate
Lactobacillus rhamnosus Lr-32Increased Superoxide Dismutase ActivityBeneficial
Moderate
Lactobacillus rhamnosus lysate powderEnhanced Growth PerformanceBeneficial
Large
Lactobacillus rhamnosus lysate powderImproved Feed UtilizationBeneficial
Large
Lactobacillus rhamnosus lysate powderIncreased Serum Lysozyme ActivityBeneficial
Moderate
Lactobacillus rhamnosus lysate powderIncreased Superoxide Dismutase ActivityBeneficial
Moderate
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