Effects of dietary supplementation with Lactobacillus acidophilus on the performance, intestinal physical barrier function, and the expression of NOD-like receptors in weaned piglets
- 2018-12-18
- PeerJ 6
- Shiqiong Wang
- Haihua Li
- C. Du
- Qian Liu
- Dongji Yang
- Longbin Chen
- Qi Zhu
- Zhixiang Wang
- PubMed: 30588399
- DOI: 10.7717/peerj.6060
Abstract
Lactobacillus supplementation is beneficial to the barrier function of the intestinal physical barrier in piglets. However, the mechanisms underlying this beneficial function remain largely unknown. Here, we investigated the effects of dietary supplementation with Lactobacillus acidophilus on the performance, intestinal physical barrier functioning, and NOD-like receptors (NLRs) expression in weaned piglets. Sixteen weaned piglets were randomly allocated to two groups. The control group received a corn-soybean basal diet, while the treatment group received the same diet adding 0.1% L. acidophilus, for 14 days. As a result, dietary L. acidophilus supplementation was found to increase the average daily gain (ADG) (P < 0.05), reduced serum diamine oxidase (DAO) activity (P < 0.05), increased the mRNA expression and protein abundance of occludin in the jejunum and ileum (P < 0.01), reduced the mRNA levels of NOD1 (P < 0.01), receptor interacting serine/threonine kinase 2 (RIPK2) (P < 0.05), nuclear factor κB (NF-κB) (P < 0.01), NLR family pyrin domain containing 3 (NLRP3) (P < 0.01), caspase-1 (P < 0.01), interleukin 1β (IL-1β) (P < 0.05) and IL-18 (P < 0.01) in the jejunum tissues of the weaned pigs. The expression of NLRP3 (P < 0.05), caspase-1 (P < 0.01), IL-1β (P < 0.05) and IL-18 (P < 0.05) was also reduced in the ileum tissues of the weaned pigs. These results showed that L. acidophilus supplementation improves the growth performance, enhances the intestinal physical barrier function, and inhibits the expression of NOD1 and NLRP3 signaling-pathway-related genes in jejunum and ileum tissues. They also suggest that L. acidophilus enhances the intestinal physical barrier functioning by inhibiting IL-1β and IL-18 pro-inflammatory cytokines via the NOD1/NLRP3 signaling pathway in weaned piglets.
Keywords: Intestinal physical barrier; Lactobacillus acidophilus; NLRs; Weaned piglet.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus acidophilus | Increased Average Daily Gain | Beneficial | Moderate |
| Lactobacillus acidophilus | Reduced Expression of Caspase-1 in Ileum | Beneficial | Large |
| Lactobacillus acidophilus | Reduced IL-18 Expression | Beneficial | Large |
| Lactobacillus acidophilus | Reduced IL-1β Expression | Beneficial | Moderate |
| Lactobacillus acidophilus | Reduced NF-κB Expression | Beneficial | Large |
| Lactobacillus acidophilus | Reduced NLRP3 Expression in Ileum | Beneficial | Moderate |
| Lactobacillus acidophilus | Reduced NOD1 Expression | Beneficial | Large |
| Lactobacillus acidophilus | Reduced RIPK2 Expression | Beneficial | Moderate |
| Lactobacillus acidophilus | Reduced Serum Diamine Oxidase Activity | Beneficial | Moderate |
| Lactobacillus acidophilus MAK32L61A | Improved Average Daily Gain | Beneficial | Moderate |
| Lactobacillus acidophilus MAK32L61A | Reduced Expression of Caspase-1 in Ileum | Beneficial | Large |
| Lactobacillus acidophilus MAK32L61A | Reduced IL-18 Expression in Ileum | Beneficial | Moderate |
| Lactobacillus acidophilus MAK32L61A | Reduced IL-18 mRNA Levels in Jejunum | Beneficial | Large |
| Lactobacillus acidophilus MAK32L61A | Reduced IL-1β Expression in Ileum | Beneficial | Moderate |
| Lactobacillus acidophilus MAK32L61A | Reduced Jejunal Caspase-1 mRNA Levels | Beneficial | Large |
| Lactobacillus acidophilus MAK32L61A | Reduced NLRP3 Expression in Ileum | Beneficial | Moderate |
| Lactobacillus acidophilus MAK32L61A | Reduced NLRP3 mRNA Levels in Jejunum | Beneficial | Large |
| Lactobacillus acidophilus MAK32L61A | Reduced RIPK2 mRNA Levels in Jejunum | Beneficial | Moderate |
| Lactobacillus acidophilus MAK32L61A | Reduced Serum Diamine Oxidase Activity | Beneficial | Moderate |
| Lactobacillus acidophilus MAK32L61A | Reduced mRNA Levels in Jejunum | Beneficial | Large |
| Lactobacillus acidophilus MAK32L61A | Reduced mRNA Levels of IL-1β in the Jejunum | Beneficial | Moderate |
| Lactobacillus acidophilus MAK32L61A | Reduced mRNA Levels of NF-κB in the Jejunum | Beneficial | Large |