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Abstract

Lactobacillus supplementation is beneficial to the barrier function of the intestinal physical barrier in piglets. However, the mechanisms underlying this beneficial function remain largely unknown. Here, we investigated the effects of dietary supplementation with Lactobacillus acidophilus on the performance, intestinal physical barrier functioning, and NOD-like receptors (NLRs) expression in weaned piglets. Sixteen weaned piglets were randomly allocated to two groups. The control group received a corn-soybean basal diet, while the treatment group received the same diet adding 0.1% L. acidophilus, for 14 days. As a result, dietary L. acidophilus supplementation was found to increase the average daily gain (ADG) (P < 0.05), reduced serum diamine oxidase (DAO) activity (P < 0.05), increased the mRNA expression and protein abundance of occludin in the jejunum and ileum (P < 0.01), reduced the mRNA levels of NOD1 (P < 0.01), receptor interacting serine/threonine kinase 2 (RIPK2) (P < 0.05), nuclear factor κB (NF-κB) (P < 0.01), NLR family pyrin domain containing 3 (NLRP3) (P < 0.01), caspase-1 (P < 0.01), interleukin 1β (IL-1β) (P < 0.05) and IL-18 (P < 0.01) in the jejunum tissues of the weaned pigs. The expression of NLRP3 (P < 0.05), caspase-1 (P < 0.01), IL-1β (P < 0.05) and IL-18 (P < 0.05) was also reduced in the ileum tissues of the weaned pigs. These results showed that L. acidophilus supplementation improves the growth performance, enhances the intestinal physical barrier function, and inhibits the expression of NOD1 and NLRP3 signaling-pathway-related genes in jejunum and ileum tissues. They also suggest that L. acidophilus enhances the intestinal physical barrier functioning by inhibiting IL-1β and IL-18 pro-inflammatory cytokines via the NOD1/NLRP3 signaling pathway in weaned piglets.

Keywords: Intestinal physical barrier; Lactobacillus acidophilus; NLRs; Weaned piglet.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus acidophilusIncreased Average Daily GainBeneficial
Moderate
Lactobacillus acidophilusReduced Expression of Caspase-1 in IleumBeneficial
Large
Lactobacillus acidophilusReduced IL-18 ExpressionBeneficial
Large
Lactobacillus acidophilusReduced IL-1β ExpressionBeneficial
Moderate
Lactobacillus acidophilusReduced NF-κB ExpressionBeneficial
Large
Lactobacillus acidophilusReduced NLRP3 Expression in IleumBeneficial
Moderate
Lactobacillus acidophilusReduced NOD1 ExpressionBeneficial
Large
Lactobacillus acidophilusReduced RIPK2 ExpressionBeneficial
Moderate
Lactobacillus acidophilusReduced Serum Diamine Oxidase ActivityBeneficial
Moderate
Lactobacillus acidophilus MAK32L61AImproved Average Daily GainBeneficial
Moderate
Lactobacillus acidophilus MAK32L61AReduced Expression of Caspase-1 in IleumBeneficial
Large
Lactobacillus acidophilus MAK32L61AReduced IL-18 Expression in IleumBeneficial
Moderate
Lactobacillus acidophilus MAK32L61AReduced IL-18 mRNA Levels in JejunumBeneficial
Large
Lactobacillus acidophilus MAK32L61AReduced IL-1β Expression in IleumBeneficial
Moderate
Lactobacillus acidophilus MAK32L61AReduced Jejunal Caspase-1 mRNA LevelsBeneficial
Large
Lactobacillus acidophilus MAK32L61AReduced mRNA Levels in JejunumBeneficial
Large
Lactobacillus acidophilus MAK32L61AReduced mRNA Levels of IL-1β in the JejunumBeneficial
Moderate
Lactobacillus acidophilus MAK32L61AReduced mRNA Levels of NF-κB in the JejunumBeneficial
Large
Lactobacillus acidophilus MAK32L61AReduced NLRP3 Expression in IleumBeneficial
Moderate
Lactobacillus acidophilus MAK32L61AReduced NLRP3 mRNA Levels in JejunumBeneficial
Large
Lactobacillus acidophilus MAK32L61AReduced RIPK2 mRNA Levels in JejunumBeneficial
Moderate
Lactobacillus acidophilus MAK32L61AReduced Serum Diamine Oxidase ActivityBeneficial
Moderate
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