Skip to main content

Abstract

A potential probiotic strain, Lactobacillus kefiranofaciens M1, was previously isolated from kefir grains, which are used to manufacture the traditional fermented drink kefir. The aim of this study was to investigate the effects of Lb. kefiranofaciens M1 on enterohemorrhagic Escherichia coli (EHEC) infection, using mice and intestinal cell models. BALB/c mice were daily administrated with either phosphate buffered saline or Lb. kefiranofaciens M1 at 2×10(8) cfu/mouse per day intragastrically for 7 d. Intragastric challenges with EHEC (2×10(9) cfu/mouse) were conducted on d 0, 4, and 7 after treatment. Administration of Lb. kefiranofaciens M1 was able to prevent EHEC infection-induced symptoms, intestinal damage, renal damage, bacterial translocation, and Shiga toxin penetration. Furthermore, the mucosal EHEC-specific IgA responses were increased after Lb. kefiranofaciens M1 administration in the EHEC-infected mouse system. Additionally, in vitro, Lb. kefiranofaciens M1 was shown to have a protective effect on Caco-2 intestinal epithelial cells and Caco-2 intestinal epithelial cell monolayers; the bacteria limited EHEC-induced cell death and reduced the loss of epithelial integrity. These findings support the potential of Lb. kefiranofaciens M1 treatment as an approach to preventing EHEC infection and its effects.

Keywords: Lactobacillus kefiranofaciens M1; enterohemorrhagic Escherichia coli; kefir; probiotic.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus kefirIncreased Mucosal EHEC-Specific IgA ResponsesBeneficial
Moderate
Lactobacillus kefirProtection Against Intestinal Epithelial InjuryBeneficial
Moderate
Lactobacillus kefirReduced EHEC Infection SymptomsBeneficial
Large
Lactobacillus kefiriIncreased Mucosal EHEC-Specific IgA ResponsesBeneficial
Moderate
Lactobacillus kefiriProtection Against Intestinal Epithelial InjuryBeneficial
Moderate
Lactobacillus kefiriReduced EHEC Infection SymptomsBeneficial
Large
⬆ Back to top
Pillser
Supplement Research and Comparison Website: evidence-based information about supplements, their benefits, potential risks, and their efficacy.
Receive updates about our products, services, sales, and special offers. Unsubscribe anytime. See our Privacy Policy for details on how we handle your information.

Join Our Community

Use support@pillser.com to get in touch.