Effects of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 on Hepatic Steatosis in Zucker Rats
- 2014-05-22
- PLoS ONE 9(5)
- J. Plaza-Díaz
- C. Gómez-Llorente
- F. Abadía-Molina
- M. J. Sáez-Lara
- L. Campaña‐Martín
- S. Muñoz-Quezada
- F. Romero
- Á. Gil
- L. Fontana
- PubMed: 24852284
- DOI: 10.1371/journal.pone.0098401
Abstract
We have previously described the safety and immunomodulatory effects of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 in healthy volunteers. The scope of this work was to evaluate the effects of these probiotic strains on the hepatic steatosis of obese rats. We used the Zucker rat as a genetic model of obesity. Zucker-Lepr(fa/fa) rats received one of three probiotic strains, a mixture of L. paracasei CNCM I-4034 and B. breve CNCM I-4035, or a placebo for 30 days. An additional group of Zucker-lean+/fa rats received a placebo for 30 days. No alterations in intestinal histology, in the epithelial, lamina propria, muscular layers of the ileal or colonic mucosa, or the submucosae, were observed in any of the experimental groups. Triacylglycerol content decreased in the liver of Zucker-Lepr(fa/fa) rats that were fed L. rhamnosus, B. breve, or the mixture of B. breve and L. paracasei. Likewise, the area corresponding to neutral lipids was significantly smaller in the liver of all four groups of Zucker-Lepr(fa/fa) rats that received probiotics than in rats fed the placebo. Zucker-Lepr(fa/fa) rats exhibited significantly greater serum LPS levels than Zucker-lean+/fa rats upon administration of placebo for 30 days. In contrast, all four groups of obese Zucker-Lepr(fa/fa) rats that received LAB strains exhibited serum LPS concentrations similar to those of Zucker-lean+/fa rats. Serum TNF-α levels decreased in the Zucker-Lepr(fa/fa) rats that received B. breve, L. rhamnosus, or the mixture, whereas L. paracasei feeding decreased IL-6 levels in the serum of Zucker-Lepr(fa/fa) rats. In conclusion, the probiotic strains reduced hepatic steatosis in part by lowering serum LPS, and had an anti-inflammatory effect in obese Zucker rats.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Bifidobacterium breve R0070 | Reduced Hepatic Steatosis | Beneficial | Moderate |
| Bifidobacterium breve R0070 | Reduced Serum LPS Levels | Beneficial | Moderate |
| Bifidobacterium breve R0070 | Reduced Serum TNF-α Levels | Beneficial | Moderate |
| Bifidobacterium breve SD5206 | Reduced Hepatic Steatosis | Beneficial | Moderate |
| Bifidobacterium breve SD5206 | Reduced Serum LPS Levels | Beneficial | Moderate |
| Bifidobacterium breve SD5206 | Reduced TNF-α Levels | Beneficial | Moderate |
| Lacticaseibacillus paracasei Lpc-37 | Reduced Hepatic Steatosis | Beneficial | Moderate |
| Lacticaseibacillus paracasei Lpc-37 | Reduced Plasma Lipopolysaccharide Levels | Beneficial | Moderate |
| Lacticaseibacillus paracasei Lpc-37 | Reduced Serum IL-6 Levels | Beneficial | Moderate |
| Lacticaseibacillus paracasei Lpc-37 | Reduced TNF-α Levels | Beneficial | Moderate |
| Lactobacillus paracasei DSM 13434 | Reduced Hepatic Steatosis | Beneficial | Moderate |
| Lactobacillus paracasei DSM 13434 | Reduced IL-6 Levels | Beneficial | Moderate |
| Lactobacillus paracasei DSM 13434 | Reduced Serum LPS Levels | Beneficial | Moderate |
| Lactobacillus paracasei DSM 13434 | Reduced Serum TNF-α Levels | Beneficial | Moderate |
| Lactobacillus paracasei HA-196 | Reduced Hepatic Steatosis | Beneficial | Moderate |
| Lactobacillus paracasei HA-196 | Reduced IL-6 Levels | Beneficial | Moderate |
| Lactobacillus paracasei HA-196 | Reduced Serum LPS Levels | Beneficial | Moderate |
| Lactobacillus paracasei HA-196 | Reduced Serum TNF-Alpha Level | Beneficial | Moderate |
| Lactobacillus paracasei LPC09 | Reduced Hepatic Steatosis | Beneficial | Moderate |
| Lactobacillus paracasei LPC09 | Reduced Plasma Lipopolysaccharide Levels | Beneficial | Moderate |
| Lactobacillus paracasei LPC09 | Reduced Serum IL-6 Levels | Beneficial | Moderate |
| Lactobacillus paracasei UALpc-04 | Reduced Hepatic Steatosis | Beneficial | Moderate |
| Lactobacillus paracasei UALpc-04 | Reduced Plasma Lipopolysaccharide Levels | Beneficial | Moderate |
| Lactobacillus paracasei UALpc-04 | Reduced Serum IL-6 Levels | Beneficial | Moderate |
| Lactobacillus paracasei UALpc-04 | Reduced TNF-α Levels | Beneficial | Moderate |
| Lactobacillus rhamnosus DSM 6594 | Reduced Hepatic Neutral Lipids | Beneficial | Large |
| Lactobacillus rhamnosus DSM 6594 | Reduced Hepatic Steatosis | Beneficial | Moderate |
| Lactobacillus rhamnosus DSM 6594 | Reduced Serum TNF-α Levels | Beneficial | Moderate |
| Lactobacillus rhamnosus LBV96 | Reduced Hepatic Steatosis | Beneficial | Moderate |
| Lactobacillus rhamnosus LBV96 | Reduced Serum LPS Levels | Beneficial | Moderate |
| Lactobacillus rhamnosus LBV96 | Reduced Serum TNF-α Levels | Beneficial | Moderate |
| Lactobacillus rhamnosus LR04 | Reduced Hepatic Steatosis | Beneficial | Moderate |
| Lactobacillus rhamnosus LR04 | Reduced Plasma Lipopolysaccharide Levels | Beneficial | Moderate |
| Lactobacillus rhamnosus LR04 | Reduced TNF-α Levels | Beneficial | Moderate |
| Lactobacillus rhamnosus LRa05 | Reduced Hepatic Steatosis | Beneficial | Moderate |
| Lactobacillus rhamnosus LRa05 | Reduced Plasma Lipopolysaccharide Levels | Beneficial | Moderate |
| Lactobacillus rhamnosus LRa05 | Reduced TNF-α Levels | Beneficial | Moderate |