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Effects of Lactococcus lactis subsp. lactis I2 with β-Glucooligosaccharides on Growth, Innate Immunity and Streptococcosis Resistance in Olive Flounder (Paralichthys olivaceus).

  • 2018-09-28
  • Journal of Microbiology and Biotechnology 28(9)
    • M. T. Hasan
    • W. Jang
    • Jin Yeong Tak
    • Bong-Joo Lee
    • K. Kim
    • Sang-woo Hur
    • Hyonsob Han
    • Bo-Seong Kim
    • Do-Huh Min
    • Shin-Kwon Kim
    • I. Kong

Abstract

To identify and quantify the effects of a combination of dietary 1 × 108 CFU/g Lactococcus lactis subsp. lactis I2 (LI2) and 0.1% β-glucooligosaccharides (BGO) on the growth and immunity of olive flounder (Paralichthys olivaceus), a feeding experiment was conducted. Flounder (14 ± 0.5 g) were divided into two groups and fed control and synbiotic feeds for 8 weeks. Investigations were carried out on growth and feed utilization, innate immunity, serum biochemical parameters, intestinal lactic acid bacterial (LAB) viability, microvillus length, and changes in the expression levels of genes encoding pro-inflammatory cytokines (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, and IL-6). Results demonstrated the synbiotic diet had significantly better (p < 0.05) responses in terms of weight gain and specific growth rate, three innate immune parameters (respiratory burst, serum lysozyme, and superoxide dismutase), intestinal LAB viability, and the relative TNF-α expression level (p < 0.05). Moreover, after challenge with Streptococcus iniae (1 × 108 CFU/ml), the synbiotically fed group exhibited significantly higher (p < 0.05) protection against streptococcosis, validating the observed changes in immune parameters and induction of the cytokine-encoding gene. Therefore, according to the results of the present study, synbiotic feed (LI2 + BGO) increased growth, modulated innate immune parameters and protected olive flounder against streptococcosis.

Keywords: Synbiotic; gene expression; growth performance; innate immunity; olive flounder.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactococcus lactis LL-23Enhanced GrowthBeneficial
Large
Lactococcus lactis LL-23Improved Innate ImmunityBeneficial
Large
Lactococcus lactis LL-23Improved Viability of Lactic Acid BacteriaBeneficial
Moderate
Lactococcus lactis LL-23Increased Resistance to Group A Streptococcal InfectionsBeneficial
Large
Lactococcus lactis LL-23Reduced Pro-Inflammatory CytokinesBeneficial
Moderate
Lactococcus lactis MJC18Elevated TNF-α Expression LevelBeneficial
Moderate
Lactococcus lactis MJC18Enhanced Protection Against Streptococcal InfectionBeneficial
Large
Lactococcus lactis MJC18Improved Growth PerformanceBeneficial
Large
Lactococcus lactis MJC18Improved Innate ImmunityBeneficial
Large
Lactococcus lactis MJC18Improved Intestinal LAB ViabilityBeneficial
Moderate
Lactococcus lactis subsp. lactisImproved Growth PerformanceBeneficial
Large
Lactococcus lactis subsp. lactisImproved Innate ImmunityBeneficial
Large
Lactococcus lactis subsp. lactisReduced Pro-Inflammatory CytokinesBeneficial
Moderate
Lactococcus lactis UALl-08Improved Growth PerformanceBeneficial
Large
Lactococcus lactis UALl-08Improved Innate ImmunityBeneficial
Large
Lactococcus lactis UALl-08Improved Intestinal LAB ViabilityBeneficial
Moderate
Lactococcus lactis UALl-08Improved Streptococcal ResistanceBeneficial
Large
Lactococcus lactis UALl-08Increased Pro-inflammatory Cytokine ExpressionBeneficial
Moderate
Lactococcus lactis VPro 34Elevated TNF-α Expression LevelBeneficial
Moderate
Lactococcus lactis VPro 34Enhanced Innate Immune FunctionBeneficial
Large
Lactococcus lactis VPro 34Improved Growth RateBeneficial
Large
Lactococcus lactis VPro 34Improved Intestinal LAB ViabilityBeneficial
Moderate
Lactococcus lactis VPro 34Improved Streptococcal ResistanceBeneficial
Large
Lactococcus lactis VPro 34Improved Weight GainBeneficial
Large

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