Effects of Pediococcus pentosaceus LI05 on immunity and metabolism in germ-free rats.

2021
Food & Function 12(11)
- Shiman Jiang
- Jiafeng Xia
- L. Lv
- Qiangqiang Wang
- Yating Li
- Qiaomai Xu
- Xiaoyuan Bian
- Jianzhong Ye
- Liya Yang
- Huiyong Jiang
- Ren Yan
- Kaicen Wang
- D. Fang
- Lanjuan Li

PubMed: 33960989
DOI: 10.1039/d0fo02530e

Study Design

Type: Clinical Trial
Population: Germ-free rats
Methods: Controlled experimental study

Animal Study

Abstract

Many Pediococcus spp. have health-promoting benefits, and Pediococcus pentosaceus LI05 is one such species that was proved to be beneficial in previous studies. Our research aimed to determine the immune and metabolic effects of P. pentosaceus LI05 on germ-free rats. Germ-free rats were gavaged with P. pentosaceus LI05 suspensions (1 × 109 CFU) for 2 weeks, and 3 weeks later, blood, spleen, intestine and liver samples were gathered for metabolome, intestine morphology, immunity, and transcriptomics analyses. Oral gavage of P. pentosaceus LI05 reduced the bodyweight of rats, which manifested as increased fecal carbohydrate concentrations, decreased intestinal fat intake and the hepatic fat synthesis gene expression, and accelerated fat-to-glycogen conversion. In addition, P. pentosaceus LI05 exhibited an anti-inflammatory ability, reducing serum proinflammatory cytokine levels and increasing intestinal subepidermal CD4+ cell levels. Furthermore, administration of P. pentosaceus LI05 increased the antimicrobial ability and enhanced the liver detoxification function. These results indicate that as a probiotic, P. pentosaceus LI05 ameliorates the hampered immune response of GF animals and improves the metabolism of fat and toxic substances.

Research Insights

Pediococcus pentosaceus KABP-041→Improved Antimicrobial Ability
Furthermore, administration of P. pentosaceus LI05 increased the antimicrobial ability and enhanced the liver detoxification function.
Effect
Beneficial
Effect size
Large
Pediococcus pentosaceus KABP-041→Improved Immune Function
P. pentosaceus LI05 exhibited an anti-inflammatory ability, reducing serum proinflammatory cytokine levels and increasing intestinal subepidermal CD4+ cell levels.
Effect
Beneficial
Effect size
Large
Pediococcus pentosaceus KABP-041→Improved Liver Function
Furthermore, administration of P. pentosaceus LI05 increased the antimicrobial ability and enhanced the liver detoxification function.
Effect
Beneficial
Effect size
Large
Pediococcus pentosaceus KABP-041→Reduced Body Weight
Oral gavage of P. pentosaceus LI05 reduced the bodyweight of rats, which manifested as increased fecal carbohydrate concentrations, decreased intestinal fat intake and the hepatic fat synthesis gene expression, and accelerated fat-to-glycogen conversion.
Effect
Beneficial
Effect size
Moderate
Pediococcus pentosaceus MAK99P15P→Improved Liver Function
Furthermore, administration of P. pentosaceus LI05 increased the antimicrobial ability and enhanced the liver detoxification function.
Effect
Beneficial
Effect size
Large
Pediococcus pentosaceus MAK99P15P→Increased Intestinal Subepidermal CD4+ Cell Levels
P. pentosaceus LI05 exhibited an anti-inflammatory ability, reducing serum proinflammatory cytokine levels and increasing intestinal subepidermal CD4+ cell levels.
Effect
Beneficial
Effect size
Moderate
Pediococcus pentosaceus MAK99P15P→Reduced Body Weight
Oral gavage of P. pentosaceus LI05 reduced the bodyweight of rats, which manifested as increased fecal carbohydrate concentrations, decreased intestinal fat intake and the hepatic fat synthesis gene expression, and accelerated fat-to-glycogen conversion.
Effect
Beneficial
Effect size
Moderate
Pediococcus pentosaceus MAK99P15P→Reduced Pro-Inflammatory Cytokines
P. pentosaceus LI05 exhibited an anti-inflammatory ability, reducing serum proinflammatory cytokine levels and increasing intestinal subepidermal CD4+ cell levels.
Effect
Beneficial
Effect size
Moderate