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Abstract

A feeding experiment was conducted to determine influences of potential probiotic Bacillus subtilis T13 (isolated from intestine of healthy sea cucumbers) on growth, immunity and disease resistance against Vibrio splendidus infection in juvenile sea cucumbers Apostichopus japonicus. Animals were fed with diets containing B. subtilis T13 at 0, 10(5), 10(7) and 10(9) CFU/g for 30 days, respectively. At the end of the growth trial, fifteen sea cucumbers from each aquarium were sampled for immune indices measurement. Then twenty sea cucumbers from each replicate were challenged with V. splendidus. Results showed that administration of B. subtilis T13 had significant effect on the specific growth rates (SGR) of sea cucumbers (P < 0.05). Phagocytosis, respiratory burst activity and total nitric oxide synthase (T-NOS) activity were significantly improved in coelomocytes of sea cucumbers fed with T13 at 10(9) CFU/g diet (P < 0.05). The highest values of the total coelomocytes counts (TCC) and superoxide dismutase (SOD) activity were found in sea cucumbers fed diet containing T13 at 10(9) CFU/g. The cumulative mortality after V. splendidus challenge decreased significantly in sea cucumbers fed with T13 at dose of 10(9) CFU/g (P < 0.05). The present study confirmed the potential beneficial effects of B. subtilis T13 as dietary probiotic in juvenile A. japonicus.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Bacillus subtilis DE111Improved PhagocytosisBeneficial
Large
Bacillus subtilis DE111Increased Nitric Oxide Synthase ActivityBeneficial
Large
Bacillus subtilis DE111Increased Respiratory Burst ActivityBeneficial
Large
Bacillus subtilis DE111Increased Superoxide Dismutase ActivityBeneficial
Large
Bacillus subtilis DE111Increased Total Coelomocyte CountBeneficial
Large
Bacillus subtilis DE111Reduced Cumulative Mortality After InfectionBeneficial
Large
Bacillus subtilis SNZ-1972Enhanced Immunity through Increased Total Nitric Oxide Synthase (T-NOS) ActivityBeneficial
Large
Bacillus subtilis SNZ-1972Improved Growth RateBeneficial
Large
Bacillus subtilis SNZ-1972Improved PhagocytosisBeneficial
Large
Bacillus subtilis SNZ-1972Increased Respiratory Burst ActivityBeneficial
Large
Bacillus subtilis SNZ-1972Increased Superoxide Dismutase ActivityBeneficial
Large
Bacillus subtilis SNZ-1972Increased Total Coelomocyte CountBeneficial
Large
Bacillus subtilis SNZ-1972Reduced Mortality After Vibrio Splendidus ChallengeBeneficial
Large

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