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Abstract

Lactococcus lactis (L. lactis)a probiotics microorganism having wide range of benefits on human health, and also protects the body from pathogenic microorganism. This study was conducted to determine the co-culture effect with the probiotic strain L. lactis (MTCC440) on Salmonella enterica serovar Typhi (S.Typhi). The existing problem was to determine the individual growth of both strains during co-culture. Growth kinetics study was performed and observed for 28 h and used to determine specific growth rate of S. Typhi under co-culture study. In growth kinetics study maximum specific growth rate (μ) of S. Typhi under monoculture and co-culture study was achieved 0.695 h-1 and 0.35 h-1 respectively. The maximum cell mass of L. lactis and S. Typhi was obtained 0.15 g/L and 0.18 g/L respectively. In co-culture study, L. lactis was found effective for the inhibition of 73% growth of S. Typhi due to lactic acid production.

Keywords: Co-culture; Growth kinetics; L. lactis; Probiotics; S. Typhi.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactococcus lactisReduced Pathogen GrowthBeneficial
Large
Lactococcus lactis L1A-23Reduced S. Typhi GrowthBeneficial
Large
Lactococcus lactis VPro 34Reduced S. Typhi GrowthBeneficial
Large
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