Effects of the probiotics on the proliferation phase in oral wound healing: In vivo study.

2025-07-01
Dental and medical problems 62(4)

PubMed: 40899389
DOI: 10.17219/dmp/195282

Study Design

Population: 60 male Sprague-Dawley® rats
Methods: Palatal wounds were induced in 60 male Sprague-Dawley® rats, which were divided into 4 groups. Probiotics, including Streptococcus salivarius K12 (BLIS K12®) and Lactobacillus reuteri (Interlac® Pro-D and Interlac®), were topically administered. The rates were sacrificed on days 3, 7 and 14 to evaluate the early, middle and late proliferation phases. Histopathological examinations assessed collagen density, angiogenesis, the re-epithelialization rate, the wound area, and the wound length.

Animal Study

Background

Probiotics, known for their anti-inflammatory properties and ability to balance the oral microbiome, show promise in enhancing wound healing, particularly through topical application, in oral healthcare.

Objectives

The main objective of the present study was to investigate the topical application of probiotics to accelerate oral wound healing, focusing on key indicators, like collagen density, angiogenesis, the reepithelization rate, the wound area, and the wound length.

Material and methods

Palatal wounds were induced in 60 male Sprague-Dawley® rats, which were divided into 4 groups. Probiotics, including Streptococcus salivarius K12 (BLIS K12®) and Lactobacillus reuteri (Interlac® Pro-D and Interlac®), were topically administered. The rates were sacrificed on days 3, 7 and 14 to evaluate the early, middle and late proliferation phases. Histopathological examinations assessed collagen density, angiogenesis, the re-epithelialization rate, the wound area, and the wound length.

Results

Probiotics showed beneficial effects on the oral wound healing indicators examined in this study. This study demonstrates the significant benefits of applying probiotics in enhancing wound healing throughout various proliferation stages. Our findings consistently highlight their positive impact across key indicators. With 3 different probiotic types, we observed improvement in all aspects of wound healing, from early to late stages.

Conclusions

The study underscores the potential of probiotics as effective agents in promoting wound repair and regeneration, offering promising avenues for enhanced clinical outcomes.

Research Insights

Supplement	Dose	Health Outcome	Effect Type	Effect Size	Source
Lactobacillus salivarius BLIS K12	—	Improved Oral Wound Healing	Beneficial	Moderate	View source Probiotics showed beneficial effects on the oral wound healing indicators examined in this study... With 3 different probiotic types, we observed improvement in all aspects of wound healing, from early to late stages.
Streptococcus salivarius BLIS K12	—	Improved Oral Wound Healing	Beneficial	Moderate	View source Probiotics showed beneficial effects on the oral wound healing indicators examined in this study... With 3 different probiotic types, we observed improvement in all aspects of wound healing, from early to late stages.
Streptococcus salivarius BLIS K12	—	Improved Skin Re-Epithelialization	Beneficial	Small	View source Histopathological examinations assessed collagen density, angiogenesis, the re-epithelialization rate, the wound area, and the wound length.
Streptococcus salivarius BLIS K12	—	Increased Angiogenesis	Beneficial	Small	View source Histopathological examinations assessed collagen density, angiogenesis, the re-epithelialization rate, the wound area, and the wound length.
Streptococcus salivarius BLIS K12	—	Increased Collagen Content	Beneficial	Small	View source Histopathological examinations assessed collagen density, angiogenesis, the re-epithelialization rate, the wound area, and the wound length.
Streptococcus salivarius BLIS K12	—	Reduced Wound Area	Beneficial	Small	View source Histopathological examinations assessed collagen density, angiogenesis, the re-epithelialization rate, the wound area, and the wound length.
Streptococcus salivarius BLIS K12	—	Reduced Wound Length	Beneficial	Small	View source Histopathological examinations assessed collagen density, angiogenesis, the re-epithelialization rate, the wound area, and the wound length.