Electrotransformation of lactobacillus acidophilus group A1.
- 1996-05
- FEMS Microbiology Letters 138(2-3)
- PubMed: 9026452
- DOI: 10.1111/J.1574-6968.1996.TB08163.X
Study Design
- Population
- Lactobacillus acidophilus strains
- Methods
- In vitro experiment
- Highly Cited
Abstract
Two strains of Lactobacillus acidophilus Group A1, the neotype ATCC 4356 and a human isolate NCFM-N2, widely used as a dietary adjunct in milk and cultured dairy products, were transformed with plasmid DNA by electroporation. The transformation characteristics exhibited by the two L acidophilus strains were found to differ markedly even though they appeared similar at the genomic level based on the DNA patterns of SmaI restriction fragments. To our knowledge, this is the first report of a consistent, reproducible transformation system of Lactobacillus acidophilus strains comprising the A1 DNA homology group.
Research Insights
| Supplement | Dose | Health Outcome | Effect Type | Effect Size | Source |
|---|