Enhancement of bile resistance by maltodextrin supplementation in Lactobacillus plantarum Lp-115.

2019-03-19
Journal of applied microbiology 126(5)

PubMed: 30790408
DOI: 10.1111/jam.14229

Study Design

Population: Lactobacillus plantarum Lp-115
Methods: Determining the OD600 value and counting the numbers of viable cells by the pour plate method; L. plantarum Lp-115 cultured in MRSB broth with maltodextrin, with 4·0% maltodextrin and bile concentrations up to 0.3%

Aims

To identify the mechanism in which way maltodextrin enhance bile tolerance in Lactobacillus plantarum Lp-115.

Methods and results

Based on determining the OD₆₀₀ value and counting the numbers of viable cells by the pour plate method, the results showed that maltodextrin could not promote the strain growth directly, but could enhance the tolerance of bile in Lp-115. The OD₆₀₀ value of L. plantarum Lp-115 cultured in MRSB broth with maltodextrin was three times higher than the control value. After supplementing the medium with 4·0% maltodextrin, the highest survival rate was observed when the bile concentration is 0.3%.

Conclusions

In summary, maltodextrin exhibited a significant improvement of bile tolerance and it could enhance cell hydrophobicity, shift the fatty acid composition of the membrane and induce the expression of a bile salt hydrolase gene (pva3) significantly.

Significance and impact of the study

This is the first report concerning the mechanism of maltodextrin enhancing the bile tolerance. This study promotes the application of maltodextrin as a choice to protect probiotic L. plantarum strains against the bile salt stress.

Research Insights

Supplement	Dose	Health Outcome	Effect Type	Effect Size	Source
Lactobacillus plantarum Lp-115	—	Improved Tolerance to Bile Salts	Beneficial	Moderate	View source maltodextrin could not promote the strain growth directly, but could enhance the tolerance of bile in Lp-115... maltodextrin exhibited a significant improvement of bile tolerance
Lactobacillus plantarum Lp-115	—	Increased Cell Hydrophobicity	Beneficial	Small	View source it could enhance cell hydrophobicity